PMID- 14961734
OWN - NLM
STAT- MEDLINE
DCOM- 20040805
LR  - 20161124
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 76
IP  - 4
DP  - 2004 Feb 15
TI  - Development of direct ELISA for the determination of 4-nonylphenol and 
      octylphenol.
PG  - 1021-7
AB  - Development of direct competitive enzyme-linked immunoadsorbent assays (ELISAs) 
      based on polyclonal and monoclonal antibodies raised against 4-n-alkylphenol 
      hapten mimics is described. A strong tendency to recognize 4-nonylphenol (NP) and 
      4-octylphenol (OP) as a total analyte amount was indicated by cross-reactivity 
      pattern established for two polyclonal antibodies. These antibodies were employed 
      for development of class-selective assays exhibiting IC(50) values around 40 
      microg.L(-1) for technical 4-NP. Specificity of the monoclonal antibody 4H6 and 
      additional two polyclonal antibodies allowed sensitive detection of linear 
      long-chain forms of 4-n-alkylphenols (4-n-AP). The assays incorporating these 
      antibodies offer a potential for detecting the minor fraction of NP/OP isomer 
      spectrum having IC(50) = 11.5 microg.L(-1) for 4-n-NP. No cross-reactivity 
      interference was indicated for linear alkylbenzene sulfonates and phenolic 
      compounds. To interpret the measured data in terms of analytical equivalents, a 
      reliable relationship between the assay responses and AP content of contaminated 
      samples should be verified and validated.
FAU - Zeravik, Jiri
AU  - Zeravik J
AD  - Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic.
FAU - Skryjova, Karla
AU  - Skryjova K
FAU - Nevorankova, Zora
AU  - Nevorankova Z
FAU - Franek, Milan
AU  - Franek M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Phenols)
RN  - 0 (octylphenol)
RN  - 79F6A2ILP5 (nonylphenol)
SB  - IM
MH  - Animals
MH  - Cross Reactions
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Mice
MH  - Phenols/*analysis/chemistry
MH  - Rabbits
EDAT- 2004/02/14 05:00
MHDA- 2004/08/06 05:00
CRDT- 2004/02/14 05:00
PHST- 2004/02/14 05:00 [pubmed]
PHST- 2004/08/06 05:00 [medline]
PHST- 2004/02/14 05:00 [entrez]
AID - 10.1021/ac030217m [doi]
PST - ppublish
SO  - Anal Chem. 2004 Feb 15;76(4):1021-7. doi: 10.1021/ac030217m.