PMID- 14962061
OWN - NLM
STAT- MEDLINE
DCOM- 20041007
LR  - 20190922
IS  - 0742-3098 (Print)
IS  - 0742-3098 (Linking)
VI  - 36
IP  - 2
DP  - 2004 Mar
TI  - Central nervous alpha1-adrenoceptor stimulation induces duodenal luminal release 
      of melatonin.
PG  - 103-8
AB  - Intracerebroventricular (i.c.v.) infusion of the alpha1-adrenoceptor agonist 
      phenylephrine elicits vagal and sympathetic neural stimulation of the bicarbonate 
      secretion by the duodenal mucosa. Melatonin originating from mucosal 
      enterochromaffin cells (EC cells) has been proposed to mediate this centrally 
      elicited stimulation. However, the release of intestinal melatonin has not been 
      studied. Rats were anesthetized with thiobarbiturate, a 12-mm segment of duodenum 
      with intact blood supply was cannulated in situ and bicarbonate secretion 
      titrated by pH-stat. The mean arterial blood pressure was continuously recorded. 
      Melatonin in the duodenal luminal perfusate was determined by high-performance 
      liquid chromatography with electrochemical detection. Intracerebroventricular 
      infusion of phenylephrine (12.2 microM/kg/hr) induced more than 10-fold increase 
      in release of melatonin into the duodenal lumen and an increase in HCO secretion 
      from 7.6 +/- 0.5 to 18.6 +/- 2.1 microEq/cm/hr. The melatonin receptor (MT2 > 
      MT1) antagonist luzindole (600 nM/kg, i.v.) almost abolished the marked rise in 
      bicarbonate secretion induced by i.c.v. phenylephrine but, in contrast, did not 
      affect the release of melatonin. These results strongly suggest that release of 
      melatonin from the mucosa mediates the duodenal secretory response to centrally 
      elicited neural stimulation.
FAU - Sjoblom, Markus
AU  - Sjoblom M
AD  - Department of Neuroscience, Division of Physiology, Uppsala University, Uppsala, 
      Sweden. markus.sjoblom@fysiologi.uu.se
FAU - Flemstrom, Gunnar
AU  - Flemstrom G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pineal Res
JT  - Journal of pineal research
JID - 8504412
RN  - 0 (Adrenergic alpha-1 Receptor Agonists)
RN  - 0 (Bicarbonates)
RN  - 0 (Receptors, Adrenergic, alpha-1)
RN  - 1WS297W6MV (Phenylephrine)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Adrenergic alpha-1 Receptor Agonists
MH  - Animals
MH  - Bicarbonates/metabolism
MH  - Brain/drug effects/*metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Duodenum/drug effects/*metabolism
MH  - Injections, Intraventricular
MH  - Melatonin/analysis/*metabolism
MH  - Phenylephrine/administration & dosage/pharmacology
MH  - Rats
MH  - Receptors, Adrenergic, alpha-1/*metabolism
MH  - Time Factors
EDAT- 2004/02/14 05:00
MHDA- 2004/10/08 09:00
CRDT- 2004/02/14 05:00
PHST- 2004/02/14 05:00 [pubmed]
PHST- 2004/10/08 09:00 [medline]
PHST- 2004/02/14 05:00 [entrez]
AID - 103 [pii]
AID - 10.1046/j.1600-079x.2003.00103.x [doi]
PST - ppublish
SO  - J Pineal Res. 2004 Mar;36(2):103-8. doi: 10.1046/j.1600-079x.2003.00103.x.