PMID- 14962258
OWN - NLM
STAT- MEDLINE
DCOM- 20040305
LR  - 20071115
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 72
IP  - 1
DP  - 2004 Jan
TI  - Dendritic cell recovery after allogeneic stem-cell transplantation in acute 
      leukemia: correlations with clinical and transplant characteristics.
PG  - 18-25
AB  - We have analyzed the kinetics of reconstitution of circulating dendritic cell 
      (DC) subsets (myeloid-DC1 and lymphoid-DC2) in 19 patients affected by acute 
      leukemia undergoing allogeneic hematopoietic stem-cell transplantation (HSCT). We 
      have found that pretransplant DC1 and DC2 were lower in leukemic patients than in 
      healthy subjects (P = 0.003 and P = 0.004, respectively) and that the number of 
      DC2 (but not DC1) infused with the graft was higher in patients receiving 
      peripheral blood stem cells (PBSC) (P = 0.03). Patients recovered to the 
      pretransplant DC1 and DC2 levels within day +60; however, a normal DC1 number was 
      reached on day +365, while DC2 remained lower than in controls up to 1 yr after 
      transplant. DC1 reconstitution did not differ significantly between patients 
      receiving bone marrow stem cells (BMSC) or PBSC, while patients receiving PBSC 
      presented increased levels of DC2 on day +30 (P = 0.008) and +100 (P = 0.047) and 
      a higher number of T lymphocytes and natural killer cells until day +365. The 
      occurrence of graft vs. host disease (GVHD) was not influenced in our cases by 
      DC1/DC2 graft composition, but patients with acute GVHD when compared with 
      patients without acute GVHD presented a significantly less rapid DC recovery (DC1 
      P = 0.03, DC2 P = 0.009 on day +30, and DC1 P = 0.012, DC2 P = 0.006 on day 
      +100). At the moment of relapse, a decrease of DC1/DC2 numbers was observed in 
      four patients and the presence of two different DC populations one with a normal 
      karyotype, and the other with the same cytogenetic abnormality as the malignant 
      clone was detected by fluorescence in situ hybridization analysis. In conclusion, 
      these observations suggest that in allogeneic HSCT recipients, DC recovery is a 
      slow process possibly contributing to the high risk of infections in the 
      post-transplant period and is possibly influenced by the source of HSC, the 
      occurrence of GVHD and relapse. Further studies are warranted to investigate the 
      significance of DC reconstitution in the transplant setting.
FAU - Porta, Matteo Della
AU  - Porta MD
AD  - Section of Hematology, Department of Biomedical Sciences, University of Ferrara, 
      Italy. sse@dns.unife.it
FAU - Rigolin, Gian Matteo
AU  - Rigolin GM
FAU - Alessandrino, Emilio Paolo
AU  - Alessandrino EP
FAU - Maiocchi, Mariangela
AU  - Maiocchi M
FAU - Malcovati, Luca
AU  - Malcovati L
FAU - Vanelli, Laura
AU  - Vanelli L
FAU - Barate, Claudia
AU  - Barate C
FAU - Rumi, Elisa
AU  - Rumi E
FAU - Ciccone, Maria
AU  - Ciccone M
FAU - Cuneo, Antonio
AU  - Cuneo A
FAU - Lazzarino, Mario
AU  - Lazzarino M
FAU - Castoldi, Gianluigi
AU  - Castoldi G
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (Antigens, CD)
RN  - 0 (Antilymphocyte Serum)
RN  - 0 (Immunosuppressive Agents)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, CD/blood
MH  - Antilymphocyte Serum/therapeutic use
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Humans
MH  - Immunosuppressive Agents/therapeutic use
MH  - Killer Cells, Natural/immunology
MH  - Leukemia, Myeloid, Acute/*therapy
MH  - Male
MH  - Middle Aged
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*therapy
MH  - Recurrence
MH  - *Stem Cell Transplantation
MH  - Transplantation Chimera
MH  - Transplantation Conditioning
MH  - Transplantation, Homologous
MH  - Whole-Body Irradiation
EDAT- 2004/02/14 05:00
MHDA- 2004/03/06 05:00
CRDT- 2004/02/14 05:00
PHST- 2004/02/14 05:00 [pubmed]
PHST- 2004/03/06 05:00 [medline]
PHST- 2004/02/14 05:00 [entrez]
AID - EJH172 [pii]
AID - 10.1046/j.0902-4441.2004.00172.x [doi]
PST - ppublish
SO  - Eur J Haematol. 2004 Jan;72(1):18-25. doi: 10.1046/j.0902-4441.2004.00172.x.