PMID- 14962496
OWN - NLM
STAT- MEDLINE
DCOM- 20040413
LR  - 20131121
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 226
IP  - 2
DP  - 2003 Dec
TI  - Immunomodulatory action of spermine and spermidine on NR8383 macrophage line in 
      various culture conditions.
PG  - 86-94
AB  - We examined the effect of spermine (SPM) and spermidine (SPD) on tumor necrosis 
      factor (TNF)alpha and monocyte chemoattractant protein-1 (MCP-1) secretion from 
      macrophages in various culture conditions, including several protocols of 
      polyamines addition and media supplemented with 0, 1 or 15% fetal bovine serum. 
      TNFalpha secretion was inhibited by SPM or SPD added 18h before stimulation in a 
      concentration-dependent manner. Their effect was directly related to the presence 
      of FBS. When SPM or SPD was added simultaneously to the stimulus, the TNFalpha 
      secretion inhibition was higher than that obtained after pre-treatment. In this 
      case, the effect was inversely proportional to the presence of FBS. The addition 
      of polyamines also inhibited the secretion of MCP-1 in NR8383 cells. We conclude 
      that SPM and SPD inhibited the secretion of inflammatory cytokines TNFalpha and 
      MCP-1 in different ways, depending on culture conditions. In any case, SPM was 
      more effective than SPD.
FAU - Perez-Cano, Francisco J
AU  - Perez-Cano FJ
AD  - Department of Physiology-Division IV, Faculty of Pharmacy, University of 
      Barcelona, Barcelona, Spain.
FAU - Franch, Angels
AU  - Franch A
FAU - Castellote, Cristina
AU  - Castellote C
FAU - Castell, Margarida
AU  - Castell M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 2FZ7Y3VOQX (Spermine)
RN  - U87FK77H25 (Spermidine)
SB  - IM
MH  - Animals
MH  - *Cell Culture Techniques/methods
MH  - Cell Line
MH  - Chemokine CCL2/immunology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages, Alveolar/*drug effects/immunology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spermidine/*pharmacology
MH  - Spermine/*pharmacology
MH  - Tumor Necrosis Factor-alpha/drug effects/immunology
EDAT- 2004/02/14 05:00
MHDA- 2004/04/14 05:00
CRDT- 2004/02/14 05:00
PHST- 2003/07/25 00:00 [received]
PHST- 2003/09/17 00:00 [accepted]
PHST- 2004/02/14 05:00 [pubmed]
PHST- 2004/04/14 05:00 [medline]
PHST- 2004/02/14 05:00 [entrez]
AID - S0008874903001990 [pii]
AID - 10.1016/j.cellimm.2003.09.009 [doi]
PST - ppublish
SO  - Cell Immunol. 2003 Dec;226(2):86-94. doi: 10.1016/j.cellimm.2003.09.009.