PMID- 14963008
OWN - NLM
STAT- MEDLINE
DCOM- 20040621
LR  - 20181130
IS  - 1524-4571 (Electronic)
IS  - 0009-7330 (Linking)
VI  - 94
IP  - 5
DP  - 2004 Mar 19
TI  - Platelet-derived growth factor-AB promotes the generation of adult bone 
      marrow-derived cardiac myocytes.
PG  - E39-45
AB  - The directed generation of cardiac myocytes from endogenous stem cells offers the 
      potential for novel therapies for cardiovascular disease. To facilitate the 
      development of such approaches, we sought to identify and exploit the pathways 
      directing the generation of cardiac myocytes from adult rodent bone marrow cells 
      (BMCs). In vitro cultures supporting the spontaneous generation of functional 
      cardiac myocytes from murine BMCs demonstrated induced expression of 
      platelet-derived growth factor (PDGF)-A and -B isoforms with alpha- and 
      beta-myosin heavy chains as well as connexin43. Supplementation of PDGF-AB 
      speeded the kinetics of myocyte development in culture by 2-fold. In a rat heart, 
      myocardial infarction pretreatment model PDGF-AB also promoted the derivation of 
      cardiac myocytes from BMCs, resulting in a significantly greater number of 
      islands of cardiac myocyte bundles within the myocardial infarction scar compared 
      with other treatment groups. However, gap junctions were detected only between 
      the cardiac myocytes receiving BMCs alone, but not BMCs injected with PDGF-AB. 
      Echocardiography and exercise testing revealed that the functional improvement of 
      hearts treated with the combination of BMCs and PDGF-AB was no greater than with 
      injections of BMCs or PDGF-AB alone. These studies demonstrated that PDGF-AB 
      enhances the generation of BMC-derived cardiac myocytes in rodent hearts, but 
      suggest that alterations in cellular patterning may limit the functional benefit 
      from the combined injection of PDGF-AB and BMCs. Strategies based on the 
      synergistic interactions of PDGF-AB and endogenous stem cells will need to 
      maintain cellular patterning in order to promote the restoration of cardiac 
      function after acute coronary occlusion.
FAU - Xaymardan, Munira
AU  - Xaymardan M
AD  - Department of Medicine, Weill Medical College of Cornell University, New York, NY 
      10021, USA.
FAU - Tang, Lilong
AU  - Tang L
FAU - Zagreda, Leze
AU  - Zagreda L
FAU - Pallante, Benedetta
AU  - Pallante B
FAU - Zheng, Jingang
AU  - Zheng J
FAU - Chazen, Joseph L
AU  - Chazen JL
FAU - Chin, Andrew
AU  - Chin A
FAU - Duignan, Inga
AU  - Duignan I
FAU - Nahirney, Patrick
AU  - Nahirney P
FAU - Rafii, Shahin
AU  - Rafii S
FAU - Mikawa, Takashi
AU  - Mikawa T
FAU - Edelberg, Jay M
AU  - Edelberg JM
LA  - eng
GR  - AG19738/AG/NIA NIH HHS/United States
GR  - AG20918/AG/NIA NIH HHS/United States
GR  - HL54128/HL/NHLBI NIH HHS/United States
GR  - HL62175/HL/NHLBI NIH HHS/United States
GR  - HL67150/HL/NHLBI NIH HHS/United States
GR  - HL67839/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040212
PL  - United States
TA  - Circ Res
JT  - Circulation research
JID - 0047103
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (platelet-derived growth factor AB)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*drug effects
MH  - Bone Marrow Transplantation
MH  - Cell Differentiation/drug effects
MH  - Computer Systems
MH  - Exercise Test
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Microscopy, Video
MH  - Myocardial Infarction/diagnostic imaging/drug therapy/pathology/therapy
MH  - Myocytes, Cardiac/*cytology
MH  - Platelet-Derived Growth Factor/genetics/pharmacology/*physiology/therapeutic use
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Inbred F344
MH  - Ultrasonography
MH  - Vascular Endothelial Growth Factor A/pharmacology
EDAT- 2004/02/14 05:00
MHDA- 2004/06/23 05:00
CRDT- 2004/02/14 05:00
PHST- 2004/02/14 05:00 [pubmed]
PHST- 2004/06/23 05:00 [medline]
PHST- 2004/02/14 05:00 [entrez]
AID - 01.RES.0000122042.51161.B6 [pii]
AID - 10.1161/01.RES.0000122042.51161.B6 [doi]
PST - ppublish
SO  - Circ Res. 2004 Mar 19;94(5):E39-45. doi: 10.1161/01.RES.0000122042.51161.B6. Epub 
      2004 Feb 12.