PMID- 14963623
OWN - NLM
STAT- MEDLINE
DCOM- 20040720
LR  - 20160512
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 379
IP  - 1
DP  - 2004 May
TI  - Determination of fifteen priority phenolic compounds in environmental samples 
      from Andalusia (Spain) by liquid chromatography-mass spectrometry.
PG  - 125-30
AB  - This work describes the optimisation of a method to determinate fifteen phenolic 
      compounds in waters, sediments and biota (green marine algae) by liquid 
      chromatography coupled to mass spectrometry (LC-MS) with atmospheric pressure 
      chemical ionisation (APCI) in the negative mode. The LC separations of the 
      studied compounds and their MS parameters were optimised in order to improve 
      selectivity and sensitivity. Separation was carried out with a C(18) column using 
      methanol and 0.005% acid acetic as mobile phase in gradient mode. The molecular 
      ion was selected for the quantitation in selective ion monitoring (SIM) mode. A 
      solid-phase extraction (SPE) method was applied in order to preconcentrate the 
      target analytes from water samples. However, extraction of the compounds from 
      sediment and biota samples was carried out by liquid-solid extraction with 
      methanol/water after studying the influence of other organic solvents. In 
      addition, a clean-up step by SPE with HLB Oasis cartridges was necessary for 
      sediments and biota. The proposed analytical methodology was validated in the 
      target environmental matrices by the analysis of spiked blank matrix samples. 
      Detection limits were 10-50 ng L(-1) for water, 1-5 microg kg(-1) for sediments 
      and 2.5-5 microg kg(-1) for biota samples. Good recoveries and precision values 
      were obtained for all matrices. This methodology has been successfully applied to 
      the analysis of incurred water, sediment and biota samples from Andalusia 
      (Spain).
FAU - Martinez Vidal, J L
AU  - Martinez Vidal JL
AD  - Department of Analytical Chemistry, University of Almeria, 04120 Almeria, Spain. 
      jlmartin@ual.es
FAU - Belmonte Vega, A
AU  - Belmonte Vega A
FAU - Garrido Frenich, A
AU  - Garrido Frenich A
FAU - Egea Gonzalez, F J
AU  - Egea Gonzalez FJ
FAU - Arrebola Liebanas, F J
AU  - Arrebola Liebanas FJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040213
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Phenols)
RN  - 0 (Water Pollutants, Chemical)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Biodiversity
MH  - Chlorophyta/chemistry
MH  - Chromatography, Liquid/methods
MH  - Environmental Monitoring/*methods
MH  - Geologic Sediments/analysis
MH  - Mass Spectrometry/methods
MH  - Phenols/*analysis/chemistry
MH  - Reproducibility of Results
MH  - Spain
MH  - Water/analysis
MH  - Water Pollutants, Chemical/*analysis
EDAT- 2004/02/14 05:00
MHDA- 2004/07/21 05:00
CRDT- 2004/02/14 05:00
PHST- 2003/10/22 00:00 [received]
PHST- 2003/12/05 00:00 [revised]
PHST- 2003/12/10 00:00 [accepted]
PHST- 2004/02/14 05:00 [pubmed]
PHST- 2004/07/21 05:00 [medline]
PHST- 2004/02/14 05:00 [entrez]
AID - 10.1007/s00216-003-2459-z [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2004 May;379(1):125-30. doi: 10.1007/s00216-003-2459-z. Epub 
      2004 Feb 13.