PMID- 14964731
OWN - NLM
STAT- MEDLINE
DCOM- 20040521
LR  - 20220331
IS  - 0090-6964 (Print)
IS  - 0090-6964 (Linking)
VI  - 32
IP  - 1
DP  - 2004 Jan
TI  - Bone marrow stromal cells (BMSCs) in bone engineering: limitations and recent 
      advances.
PG  - 160-5
AB  - Bone marrow stromal cells (BMSCs) have been isolated for the first time by 
      Friedenstein et al. and since then have been considered the progenitor cells for 
      the skeletal tissues. Indeed BMSCs are clonogenic, fibroblastic in shape, and can 
      differentiate along multiple lineages such as osteoblasts, chondrocytes, 
      adipocytes, and hematopoiesis-supportive stroma. When implanted in vivo on a 
      three-dimensional bioceramic scaffold into immunocompromised mice, BMSCs form 
      bone and hematopoiesis-supportive stroma. The ease of harvest from a donor bone 
      marrow together with the ability to form bone in vivo make BMSCs ideal for 
      clinical applications. Thus, ex vivo expanded BMSCs have been employed, first in 
      large animal models, then in human clinical trials, to repair large bone 
      segmental defects. Further investigation of the expanded BMSC population led to 
      the observation that in vitro expansion appears a limiting passage: cells tend to 
      senesce and lose their multidifferentiation potential with time in culture. To 
      overcome these limitations, two approaches have been proposed: (1) identification 
      of the appropriate culture conditions to prevent senescence by possibly selecting 
      a subpopulation with stem cell characteristics, and (2) engineering of the cells 
      by transfection with the telomerase gene to prevent cells from telomere 
      shortening and consequent aging.
FAU - Derubeis, Anna R
AU  - Derubeis AR
AD  - Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
FAU - Cancedda, Ranieri
AU  - Cancedda R
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Ann Biomed Eng
JT  - Annals of biomedical engineering
JID - 0361512
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*physiology
MH  - Bone Marrow Transplantation/*methods/trends
MH  - Bone and Bones/*cytology/*physiology
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation/*physiology
MH  - Humans
MH  - Osteogenesis/*physiology
MH  - Stromal Cells/cytology/physiology
MH  - Tissue Engineering/*methods/*trends
RF  - 47
EDAT- 2004/02/18 05:00
MHDA- 2004/05/22 05:00
CRDT- 2004/02/18 05:00
PHST- 2004/02/18 05:00 [pubmed]
PHST- 2004/05/22 05:00 [medline]
PHST- 2004/02/18 05:00 [entrez]
AID - 10.1023/b:abme.0000007800.89194.95 [doi]
PST - ppublish
SO  - Ann Biomed Eng. 2004 Jan;32(1):160-5. doi: 10.1023/b:abme.0000007800.89194.95.