PMID- 14966192
OWN - NLM
STAT- MEDLINE
DCOM- 20040611
LR  - 20240109
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 75
IP  - 5
DP  - 2004 May
TI  - Streptococcus pyogenes and Lactobacillus rhamnosus differentially induce 
      maturation and production of Th1-type cytokines and chemokines in human 
      monocyte-derived dendritic cells.
PG  - 764-71
AB  - Dendritic cells (DCs) are the most efficient antigen-presenting cells and thus, 
      have a major role in regulating host immune responses. In the present study, we 
      have analyzed the ability of Gram-positive, pathogenic Streptococcus pyogenes and 
      nonpathogenic Lactobacillus rhamnosus to induce the maturation of human 
      monocyte-derived DCs. Stimulation of DCs with S. pyogenes resulted in strong 
      expression of DC costimulatory molecules CD80, CD83, and CD86 accompanied with a 
      T helper cell type 1 (Th1) cytokine and chemokine response. S. pyogenes also 
      induced interleukin (IL)-2 and IL-12 production at mRNA and protein levels. In 
      addition, IL-23 and IL-27 subunits p40, p19, p28, and EBI3 were induced at mRNA 
      level. In contrast, L. rhamnosus-stimulated DCs showed only moderate expression 
      of costimulatory molecules and produced low levels of cytokines and chemokines. 
      Furthermore, no production of IL-2 or IL-12 family cytokines was detected. 
      Bacteria-induced DC maturation and especially cytokine and chemokine production 
      were reduced when bacteria were heat-inactivated. Our results show that human 
      monocyte-derived DCs respond differently to different Gram-positive bacteria. 
      Although pathogenic S. pyogenes induced a strong Th1-type response, stimulation 
      with nonpathogenic L. rhamnosus resulted in development of semi-mature DCs 
      characterized by moderate expression of costimulatory molecules and low cytokine 
      production.
FAU - Veckman, Ville
AU  - Veckman V
AD  - Department of Microbiology, National Public Health Institute, Helsinki, Finland. 
      ville.veckman@ktl.fi
FAU - Miettinen, Minja
AU  - Miettinen M
FAU - Pirhonen, Jaana
AU  - Pirhonen J
FAU - Siren, Jukka
AU  - Siren J
FAU - Matikainen, Sampsa
AU  - Matikainen S
FAU - Julkunen, Ilkka
AU  - Julkunen I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040213
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cell Differentiation/immunology
MH  - Chemokines/biosynthesis/genetics
MH  - Cytokines/*biosynthesis/genetics
MH  - Dendritic Cells/cytology/*immunology
MH  - Gene Expression Regulation/immunology
MH  - Gram-Positive Bacteria/immunology/pathogenicity
MH  - Humans
MH  - Lactobacillus/*immunology/pathogenicity
MH  - Monocytes/cytology
MH  - RNA, Messenger/biosynthesis
MH  - Streptococcus pyogenes/*immunology/pathogenicity
EDAT- 2004/02/18 05:00
MHDA- 2004/06/21 10:00
CRDT- 2004/02/18 05:00
PHST- 2004/02/18 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/02/18 05:00 [entrez]
AID - jlb.1003461 [pii]
AID - 10.1189/jlb.1003461 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2004 May;75(5):764-71. doi: 10.1189/jlb.1003461. Epub 2004 Feb 13.