PMID- 14966928
OWN - NLM
STAT- MEDLINE
DCOM- 20040504
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 10
IP  - 4
DP  - 2004 Feb 15
TI  - Effects of lipopolysaccharides stimulated Kupffer cells on activation of rat 
      hepatic stellate cells.
PG  - 610-3
AB  - AIM: To study the effects of Kupffer cell-conditioned medium (KCCM) derived from 
      lipopolysaccharide (LPS) treatment on proliferation of rat hepatic stellate cells 
      (HSC). METHODS: HSC and Kupffer cells were isolated from the liver of Wistar rats 
      by in situ perfusion with pronase and collagenase and density gradient 
      centrifugation with Nycodenz and cultured. KCCM was prepared and 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric 
      assay was used to detect HSC proliferation. The content of type IV collagen and 
      laminin secreted by HSC in the HSC-conditioned medium was determined by 
      radioimmunoassay. TGF-beta(1) production in the KCCM was detected by 
      enzyme-linked immunosorbent assay (ELISA). RESULTS: HSC and Kupffer cells 
      isolated had high purity. One microgram per mililiter LPS-activated KCCM and 
      unstimulated KCCM could significantly promote HSC proliferation [0.132+/-0.005 
      and 0.123+/-0.008 vs control group (0.100+/-0.003), P<0.01], and there was a 
      difference between them (P<0.05). Ten microgram per mililiter LPS-activated KCCM 
      (0.106+/-0.010) was unable to promote HSC proliferation (P>0.05). Adding 
      anti-TGF-beta(1) antibodies could suppress the proliferation promoted by 
      unstimulated KCCM and LPS (1 microg/ml)-activated KCCM (0.109+/-0.009 vs 
      0.123+/-0.008, 0.115+/-0.008 vs 0.132+/-0.005, P<0.01). LPS (1 microg/ml or 10 
      microg/ml) could not promote HSC proliferation immediately (0.096+/-0.003 and 
      0.101+/-0.004 vs 0.100+/-0.003, P>0.05). There was a parallel behavior between 
      HSC proliferation and increased ECM level. One microgram per mililiter 
      LPS-activated KCCM contained a larger amount of TGF-beta(1) than unstimulated 
      KCCM. CONCLUSION: The technique for isolation of HSC and Kupffer cells described 
      here is simple and reliable. KCCM stimulated by LPS may promote HSC proliferation 
      and collagen accumulation, which are associated with hepatic fibrogenesis.
FAU - Zhang, Xin
AU  - Zhang X
AD  - Department of Pathology and Pathophysiology, Basic Medical School, Southeast 
      University, Nanjing 210009, Jiangsu Province, China.
FAU - Yu, Wei-Ping
AU  - Yu WP
FAU - Gao, Lei
AU  - Gao L
FAU - Wei, Kai-Bin
AU  - Wei KB
FAU - Ju, Jiu-Long
AU  - Ju JL
FAU - Xu, Jia-Zhang
AU  - Xu JZ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Collagen Type IV)
RN  - 0 (Laminin)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Cell Communication/drug effects
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Collagen Type IV/biosynthesis/metabolism
MH  - Kupffer Cells/*cytology/*drug effects
MH  - Laminin/metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Liver/*cytology/metabolism
MH  - Rats
MH  - Transforming Growth Factor beta/metabolism
MH  - Transforming Growth Factor beta1
PMC - PMC4716991
EDAT- 2004/02/18 05:00
MHDA- 2004/05/05 05:00
PMCR- 2004/02/15
CRDT- 2004/02/18 05:00
PHST- 2004/02/18 05:00 [pubmed]
PHST- 2004/05/05 05:00 [medline]
PHST- 2004/02/18 05:00 [entrez]
PHST- 2004/02/15 00:00 [pmc-release]
AID - 10.3748/wjg.v10.i4.610 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2004 Feb 15;10(4):610-3. doi: 10.3748/wjg.v10.i4.610.