PMID- 14967382
OWN - NLM
STAT- MEDLINE
DCOM- 20040324
LR  - 20061115
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 81
IP  - 2
DP  - 2004 Feb
TI  - Fast multicolor primed in situ protocol for chromosome identification in isolated 
      cells may be used for human oocytes and polar bodies.
PG  - 408-15
AB  - OBJECTIVE: To present and evaluate the use of a new ultra-fast multicolor primed 
      in situ (PRINS) procedure for karyotyping human oocytes and first polar bodies. 
      DESIGN: In situ chromosomal identification on isolated cells, using combinations 
      of specific primers for chromosomes 1, 7, 9, 16, and 18 and fluorescent 
      nucleotides. SETTING: Sixteen unfertilized oocytes were obtained from women 
      participating in an IVF program. PATIENT(S): Five patients undergoing an IVF-ET. 
      INTERVENTION(S): In vitro unfertilized oocytes were fixed on slides, and 
      sequential PRINS reactions were performed on each preparation. MAIN OUTCOME 
      MEASURE(S): Ultrarapid in situ identification of three or four chromosomes on 
      oocyte and polar body chromosome spreads. RESULT(S): On the basis of the direct 
      in situ mixing of the colors of fluorochromes (FITC, TRITC, Cascade Blue) that 
      were incorporated in sequential PRINS reactions, this method allows rapid and 
      efficient labeling of three or four individual chromosomes. Each PRINS reaction 
      consists of a unique 4- to 6-minute step for both in situ annealing and 
      elongation. The procedure can be combined with fluorescence in situ hybridization 
      (FISH) reactions. CONCLUSION(S): By simplifying the multicolor PRINS procedure, 
      this new protocol should facilitate the use and adaptation of PRINS to chromosome 
      screening. This approach could be used in parallel or in combination with FISH 
      for efficient aneuploidy assessment on isolated cells.
FAU - Pellestor, Franck
AU  - Pellestor F
AD  - Institut of Human Genetics and Clinique St. Roch. Montpellier, France. 
      franck.pellestor@igh.cnrs.fr
FAU - Anahory, Tal
AU  - Anahory T
FAU - Andreo, Brigitte
AU  - Andreo B
FAU - Regnier-Vigouroux, Gilles
AU  - Regnier-Vigouroux G
FAU - Soulie, Jean Pierre
AU  - Soulie JP
FAU - Baudouin, Magalie
AU  - Baudouin M
FAU - Demaille, Jacques
AU  - Demaille J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes, Human/genetics/*ultrastructure
MH  - Embryo Transfer
MH  - Female
MH  - Fertilization in Vitro
MH  - Haploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Oocytes/*cytology/ultrastructure
EDAT- 2004/02/18 05:00
MHDA- 2004/03/25 05:00
CRDT- 2004/02/18 05:00
PHST- 2002/12/30 00:00 [received]
PHST- 2003/08/15 00:00 [revised]
PHST- 2003/08/15 00:00 [accepted]
PHST- 2004/02/18 05:00 [pubmed]
PHST- 2004/03/25 05:00 [medline]
PHST- 2004/02/18 05:00 [entrez]
AID - S0015028203028802 [pii]
AID - 10.1016/j.fertnstert.2003.08.014 [doi]
PST - ppublish
SO  - Fertil Steril. 2004 Feb;81(2):408-15. doi: 10.1016/j.fertnstert.2003.08.014.