PMID- 14968124
OWN - NLM
STAT- MEDLINE
DCOM- 20040506
LR  - 20230216
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 84
IP  - 4
DP  - 2004 Apr
TI  - Matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases mRNA 
      transcripts in the bronchial secretions of asthmatics.
PG  - 418-24
AB  - Asthma is a chronic inflammatory disease characterized by profound extracellular 
      matrix changes referred to as bronchial remodelling. In this study, we evaluated 
      matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) mRNA 
      expression in bronchial secretions of asthmatics and correlated MMPs modulations 
      with the lung function as a reflection of the bronchial extracellular matrix 
      remodelling. Quantitative RT-PCR was performed on cell pellets obtained from 
      induced sputum in order to detect the mRNAs for MMP-1, -2, -3, -8, -9, -12, -13 
      TIMP-1, -2, while semiquantitative RT-PCR was performed to assess the expression 
      of MMP-7, monocyte chemoattractant protein-1 (MCP-1) and transforming growth 
      factor-beta(1) (TGF-beta(1)). The mRNA transcripts for MMP-1, TIMP-1 and monocyte 
      chemoattractant protein-1 (MCP-1) were increased in cell pellets of induced 
      sputum from asthmatics when compared to controls (P<0.05), and the intensity of 
      MMP-1 mRNA expression inversely correlated with the FEV(1) in asthmatics 
      (r=-0.49, P<0.05). The MMP-1 mRNA/TIMP-1 mRNA ratio correlated with the levels of 
      MCP-1 mRNA in asthmatics (r=0.47, P<0.05). There were no differences between the 
      groups with respect to mRNA coding for MMP-2, -3, -7, -8, -9, -12, -13, -14, 
      TIMP-2 and TGF-beta(1). We conclude that cells contained in the bronchial 
      secretions from asthmatics express higher amounts of mRNA for MMP-1 and TIMP-1, 
      perhaps related to an increased expression of MCP-1, which might contribute to 
      the extracellular matrix changes observed during airway remodelling.
FAU - Cataldo, Didier D
AU  - Cataldo DD
AD  - Department of Pneumology, University of Liege, CHU Sart-Tilman, Liege 4000, 
      Belgium. Didier.Cataldo@ulg.ac.be
FAU - Gueders, Maud
AU  - Gueders M
FAU - Munaut, Carine
AU  - Munaut C
FAU - Rocks, Natacha
AU  - Rocks N
FAU - Bartsch, Pierre
AU  - Bartsch P
FAU - Foidart, Jean-Michel
AU  - Foidart JM
FAU - Noel, Agnes
AU  - Noel A
FAU - Louis, Renaud
AU  - Louis R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Asthma/*metabolism
MH  - Bronchi/*metabolism
MH  - Cell Count
MH  - Chemokine CCL2/genetics
MH  - Female
MH  - Forced Expiratory Volume
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 2/genetics
MH  - Matrix Metalloproteinase 9/genetics
MH  - Matrix Metalloproteinases/*genetics
MH  - Middle Aged
MH  - RNA, Messenger/*analysis
MH  - Tissue Inhibitor of Metalloproteinase-1/*genetics
EDAT- 2004/02/18 05:00
MHDA- 2004/05/07 05:00
CRDT- 2004/02/18 05:00
PHST- 2004/02/18 05:00 [pubmed]
PHST- 2004/05/07 05:00 [medline]
PHST- 2004/02/18 05:00 [entrez]
AID - S0023-6837(22)02230-9 [pii]
AID - 10.1038/labinvest.3700063 [doi]
PST - ppublish
SO  - Lab Invest. 2004 Apr;84(4):418-24. doi: 10.1038/labinvest.3700063.