PMID- 14968437
OWN - NLM
STAT- MEDLINE
DCOM- 20040330
LR  - 20071114
IS  - 0270-4137 (Print)
IS  - 0270-4137 (Linking)
VI  - 58
IP  - 4
DP  - 2004 Mar 1
TI  - Effects of transforming growth factor-beta1 on parathyroid hormone-related 
      protein mRNA expression and protein secretion in canine prostate epithelial, 
      stromal, and carcinoma cells.
PG  - 366-73
AB  - BACKGROUND: Bone metastases of prostate carcinoma are associated with 
      osteoblastic metastases. Tumor-derived factors, such as parathyroid 
      hormone-related protein (PTHrP), may promote the development of osteoblastic 
      metastases. We examined the effect of transforming growth factor-beta1 (TGF beta 
      1) on PTHrP mRNA expression and PTHrP secretion in normal canine prostate 
      epithelial cells (PEC) and stromal cells (PSC), and in canine prostate carcinoma 
      cells (PCC). METHODS: Primary cultures of PEC, PSC, and PCC were produced. The 
      effect of TGF beta 1 on PTHrP mRNA expression was measured by Northern blot, and 
      secretion of PTHrP into culture medium was measured by immunoradiometric assay 
      (IRMA). Degradation of recombinant-human PTHrP (rhPTHrP) (1-84) inoculated in 
      prostate cell cultures was measured over 24 hr. Arginine esterase (AE) activity 
      in tissue and conditioned medium was also measured. RESULTS: TGF beta 1 increased 
      PTHrP mRNA expression in a time- and dose-dependent manner in PEC and in PCC. TGF 
      beta 1 decreased PTHrP mRNA in PSC. TGF beta 1 significantly increased PTHrP 
      secretion (P < or = 0.05) into PEC but not PSC conditioned medium. rhPTHrP was 
      significantly (P < or = 0.05) degraded in PEC conditioned medium as compared to 
      PSC conditioned medium. AE activity was present in prostate and prostate 
      carcinoma tissue, but not in conditioned medium from PEC or PSC. CONCLUSIONS: TGF 
      beta 1 increased PTHrP mRNA expression in canine PEC and PCC, and decreased 
      expression in PSC. This regulatory pathway may be important in the pathogenesis 
      of osteoblastic metastases.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Sellers, Rani S
AU  - Sellers RS
AD  - The Department of Veterinary Biosciences, The Ohio State University, Columbus, 
      43210, USA.
FAU - LeRoy, Bruce E
AU  - LeRoy BE
FAU - Blomme, Eric A G
AU  - Blomme EA
FAU - Tannehill-Gregg, Sarah
AU  - Tannehill-Gregg S
FAU - Corn, Stephanie
AU  - Corn S
FAU - Rosol, Thomas J
AU  - Rosol TJ
LA  - eng
GR  - CA-09338/CA/NCI NIH HHS/United States
GR  - CA-77911/CA/NCI NIH HHS/United States
GR  - CA79110-01/CA/NCI NIH HHS/United States
GR  - RR-00168/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Prostate
JT  - The Prostate
JID - 8101368
RN  - 0 (Parathyroid Hormone-Related Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Dogs
MH  - Epithelial Cells/cytology/drug effects/pathology/physiology
MH  - Gene Expression Regulation/*drug effects
MH  - Male
MH  - Parathyroid Hormone-Related Protein/*genetics
MH  - Prostate/cytology/*physiology
MH  - Prostatic Neoplasms/genetics/pathology/*physiopathology
MH  - RNA, Messenger/*genetics
MH  - Stromal Cells/*cytology/drug effects/pathology/physiology
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 2004/02/18 05:00
MHDA- 2004/03/31 05:00
CRDT- 2004/02/18 05:00
PHST- 2004/02/18 05:00 [pubmed]
PHST- 2004/03/31 05:00 [medline]
PHST- 2004/02/18 05:00 [entrez]
AID - 10.1002/pros.20029 [doi]
PST - ppublish
SO  - Prostate. 2004 Mar 1;58(4):366-73. doi: 10.1002/pros.20029.