PMID- 14968921
OWN - NLM
STAT- MEDLINE
DCOM- 20040927
LR  - 20061115
IS  - 0120-4157 (Print)
IS  - 0120-4157 (Linking)
VI  - 23
IP  - 4
DP  - 2003 Dec
TI  - Molecular cloning and characterization of two hsp 70 homologous genes from the 
      dimorphic fungus Paracoccidioides brasiliensis.
PG  - 424-36
AB  - Paracoccidioides brasiliensis, a dimorphic fungus, is the etiologic agent of 
      Paracoccidioidomycosis (PCM), one of the most important systemic mycosis in Latin 
      America. Two genes (2.2 and 1DB5) were cloned, characterized and sequenced; they 
      showed homology with members of hsp70 gene family. By using several probe 
      fragments derived from these genes, levels of expression for each gene were 
      determined by Northern blot during transition to the yeast phase. The highest 
      level of hsp70 transcript occurred between 30 min to 6 hours after temperature 
      shift, with significant reduction after 36-48 hours. However, after 72 hours, the 
      level of the transcription increased until yeast phase was reached. As a response 
      to temperature increase, hsp 70 genes are expressed during the transition phase 
      and possibly play a role in the differentiation process.
FAU - Florez, Alvaro M
AU  - Florez AM
AD  - Corporacion Ciencias Basicas Biomedicas, Universidad de Antioquia, Medellin, 
      Colombia.
FAU - Oviedo, Adona
AU  - Oviedo A
FAU - Cardona, Astrid
AU  - Cardona A
FAU - Herrera, Monica
AU  - Herrera M
FAU - Garcia, Edwin
AU  - Garcia E
FAU - Restrepo, Angela
AU  - Restrepo A
FAU - McEwen, Juan G
AU  - McEwen JG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Colombia
TA  - Biomedica
JT  - Biomedica : revista del Instituto Nacional de Salud
JID - 8205605
RN  - 0 (HSP70 Heat-Shock Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Cloning, Molecular/methods
MH  - Genes, Fungal/*genetics
MH  - HSP70 Heat-Shock Proteins/*genetics
MH  - Molecular Sequence Data
MH  - Paracoccidioides/*genetics
EDAT- 2004/02/19 05:00
MHDA- 2004/09/28 05:00
CRDT- 2004/02/19 05:00
PHST- 2004/02/19 05:00 [pubmed]
PHST- 2004/09/28 05:00 [medline]
PHST- 2004/02/19 05:00 [entrez]
PST - ppublish
SO  - Biomedica. 2003 Dec;23(4):424-36.