PMID- 14969820
OWN - NLM
STAT- MEDLINE
DCOM- 20040607
LR  - 20041117
IS  - 1368-8375 (Print)
IS  - 1368-8375 (Linking)
VI  - 40
IP  - 4
DP  - 2004 Apr
TI  - Numerical aberrations of chromosomes 8, 9, 11, and 17 in squamous cell carcinoma 
      of the pharynx and larynx: a fluorescence in situ hybridization and DNA flow 
      cytometric analysis of 50 cases.
PG  - 409-17
AB  - Squamous cell carcinoma of the pharynx and larynx (SCCPL) is a genetically 
      complex disease and is frequently associated with nonrandom chromosomal 
      alterations. Fifty primary SCC of the pharynx (oropharynx, n=11): see and 
      hypopharynx, n=11) and larynx ( n=28) were examined for numerical aberrations of 
      chromosomes 8, 9, 11, and 17 with a panel of chromosome-specific repetitive DNA 
      probes by fluorescence in situ hybridization (FISH). DNA ploidy analysis was also 
      performed by flow cytometry (FCM). Aneusomic copy numbers of chromosomes 8, 9, 
      11, and 17 were discovered in 66%, 68%, 68% and 78% of tumors, respectively. FCM 
      showed abnormal DNA content in 74% of cases (mean DNA index=1.69). Polysomy was 
      the main finding in both DNA-aneuploid and DNA-diploid tumors (64.5% of cases). 
      Numerical aberrations of chromosomes 8 and 11 correlated to DNA ploidy by FCM (P< 
      0.05). Aneusomy was present in 69.23% of DNA-diploid tumors. Marked intratumoral 
      and intertumoral chromosomal heterogeneity was noted between individual tumors, 
      suggesting a notable heterogeneity in aneuploid and diploid cell populations. 
      Interphase FISH can be used to study important cytogenetic changes which occur 
      during the development of SCC of the pharynx and larynx.
FAU - Hardisson, David
AU  - Hardisson D
AD  - Department of Pathology, Hospital Universitario La Paz, Autonomous University of 
      Madrid, Paseo de la Castellana 261, E-28046 Madrid, Spain. 
      dhardisson.hulp@salud.madrid.org
FAU - Alvarez-Marcos, Cesar
AU  - Alvarez-Marcos C
FAU - Salas-Bustamante, Ana
AU  - Salas-Bustamante A
FAU - Alonso-Guervos, Marta
AU  - Alonso-Guervos M
FAU - Sastre, Noelia
AU  - Sastre N
FAU - Sampedro, Andres
AU  - Sampedro A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Oral Oncol
JT  - Oral oncology
JID - 9709118
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - DNA, Neoplasm/genetics
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Pharyngeal Neoplasms/*genetics/pathology
MH  - Ploidies
EDAT- 2004/02/19 05:00
MHDA- 2004/06/21 10:00
CRDT- 2004/02/19 05:00
PHST- 2003/09/04 00:00 [received]
PHST- 2003/09/18 00:00 [accepted]
PHST- 2004/02/19 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/02/19 05:00 [entrez]
AID - S1368837503002136 [pii]
AID - 10.1016/j.oraloncology.2003.09.008 [doi]
PST - ppublish
SO  - Oral Oncol. 2004 Apr;40(4):409-17. doi: 10.1016/j.oraloncology.2003.09.008.