PMID- 14970589
OWN - NLM
STAT- MEDLINE
DCOM- 20040319
LR  - 20190901
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 246
DP  - 2004
TI  - Adenovirus-mediated gene delivery to dendritic cells.
PG  - 139-54
AB  - Dendritic cells (DCs) are "professional" antigen-presenting cells (APCs) that are 
      uniquely capable of activating and instructing a naive immune system to mount a 
      specific cellular and humoral response. Recognition of this crucial function 
      makes the development of technologies for DC-based immuno-therapies a priority 
      for the treatment of a wide variety of diseases. The most immediate impact of 
      this emerging technology will be in the treatment of cancer and the development 
      of third generation vaccines to protect against viral and intracellular 
      pathogens. In addition to elicitation of immune responses, DCs also function to 
      maintain tolerance to "self." Once the biological basis for this important 
      function is understood, future applications of DC-based immuotherapies may be 
      developed to ameliorate autoimmune diseases or enhance acceptance of transplanted 
      organs. The feasibility of "engineering" the function of DCs has been realized by 
      recent advances in ex vivo methodologies that allow selective DC propagation, 
      antigen loading, and genetic modification in vitro for subsequent therapeutic 
      transfer into the host. Ultimately, the ability to genetically modify these cells 
      will allow us to design DC-mediated interventions that will direct predictable 
      control of either immune activation or tolerance in vivo.
FAU - Timares, Laura
AU  - Timares L
AD  - Departments of Dermatology, Cell Biology, and Pathology, and the Gene Therapy 
      Center, University of Alabama at Birmingham, Birmingham, AL, USA.
FAU - Douglas, Joanne T
AU  - Douglas JT
FAU - Tillman, Bryan W
AU  - Tillman BW
FAU - Krasnykh, Victor
AU  - Krasnykh V
FAU - Curiel, David T
AU  - Curiel DT
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Apoptosis
MH  - Dendritic Cells/*metabolism
MH  - Flow Cytometry
MH  - *Gene Transfer Techniques
MH  - *Genetic Vectors
MH  - Humans
MH  - Lymphocyte Activation
MH  - Mice
EDAT- 2004/02/19 05:00
MHDA- 2004/03/20 05:00
CRDT- 2004/02/19 05:00
PHST- 2004/02/19 05:00 [pubmed]
PHST- 2004/03/20 05:00 [medline]
PHST- 2004/02/19 05:00 [entrez]
AID - 1-59259-650-9:139 [pii]
AID - 10.1385/1-59259-650-9:139 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2004;246:139-54. doi: 10.1385/1-59259-650-9:139.