PMID- 14970599
OWN - NLM
STAT- MEDLINE
DCOM- 20040319
LR  - 20190901
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 246
DP  - 2004
TI  - Delivery using herpes simplex virus: an overview.
PG  - 257-99
AB  - The human herpesviruses represent excellent candidate viruses for several types 
      of gene vector applications. As a class, they are large DNA viruses with the 
      potential to accommodate large or multiple transgene cassettes, and they have 
      evolved to persist in a lifelong nonintegrated latent state without causing 
      disease in the immune-competent host. Among the herpesviruses, herpes simplex 
      virus type 1 (HSV-1) is an attractive vehicle because in natural infection, the 
      virus establishes latency in neurons, a state in which viral genomes may persist 
      for the life of the host as intranuclear episomal elements. The natural lifelong 
      persistence of latent genomes in trigeminal ganglia (TG) without the development 
      of sensory loss or histologic damage to the ganglion attests to the effectiveness 
      of these natural latency mechanisms. Although the wild-type virus may be 
      reactivated from latency under the influence of a variety of stresses, completely 
      replication defective viruses can be constructed that retain the ability to 
      establish persistent quiescent genomes in neurons, but that are unable to 
      subsequently reactivate in the nervous system. These persistent genomes are 
      devoid of lytic gene expression, but retain the ability to express 
      latency-associated transcripts (LATs).
FAU - Goins, William F
AU  - Goins WF
AD  - Department of Molecular Genetics and Biochemistry, University of Pittsburgh 
      School of Medicine, Pittsburgh, PA, USA.
FAU - Wolfe, Darren
AU  - Wolfe D
FAU - Krisky, David M
AU  - Krisky DM
FAU - Bai, Qing
AU  - Bai Q
FAU - Burton, Ed A
AU  - Burton EA
FAU - Fink, David J
AU  - Fink DJ
FAU - Glorioso, Joseph C
AU  - Glorioso JC
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Defective Viruses/genetics/physiology
MH  - *Gene Transfer Techniques
MH  - Genetic Vectors
MH  - Genome, Viral
MH  - Herpes Simplex/physiopathology/virology
MH  - Humans
MH  - Simplexvirus/*genetics/physiology
MH  - Virus Latency
RF  - 286
EDAT- 2004/02/19 05:00
MHDA- 2004/03/20 05:00
CRDT- 2004/02/19 05:00
PHST- 2004/02/19 05:00 [pubmed]
PHST- 2004/03/20 05:00 [medline]
PHST- 2004/02/19 05:00 [entrez]
AID - 1-59259-650-9:257 [pii]
AID - 10.1385/1-59259-650-9:257 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2004;246:257-99. doi: 10.1385/1-59259-650-9:257.