PMID- 14970610
OWN - NLM
STAT- MEDLINE
DCOM- 20040319
LR  - 20190901
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 246
DP  - 2004
TI  - Lentiviral transduction of human dendritic cells.
PG  - 451-9
AB  - Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that play a 
      pivotal role in stimulating antigen-specific T cells in vivo. The cardinal 
      properties of DCs are: the ability to take up, process, and present antigens; (2) 
      the ability to migrate through different tissues into lymphoid organs; and (3) 
      the ability to interact with and stimulate T cells (3). Because of their unique 
      capability of generating primary CD4+ and CD8+ T-cell responses, DCs are of 
      particular interest for immunotherapeutic approaches to infectious disease and 
      cancer. There are many ways to load DCs with antigen and to subsequently induce 
      specific immune responses in vivo and in vitro. One strategy relies on 
      genetically modified peripheral blood mononuclear cell (PBMC)-derived DCs to 
      establish expression of a gene that encodes a specific antigen. In contrast to 
      pulsing DCs with antigenic peptides or proteins, genetic engineering of DCs has 
      the advantages of providing multiple epitopes for major histocompatibility class 
      (MHC) class I-and MHC class II-restricted immune responses as well as a 
      continuous supply of antigen for presentation alone or together with 
      immunomodulatory proteins (cytokines, for example).
FAU - Schroers, Roland
AU  - Schroers R
AD  - Department of Internal Medicine, University of Gottingen, Gottingen, Germany.
FAU - Chen, Si-Yi
AU  - Chen SY
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Cell Line
MH  - Dendritic Cells/*metabolism
MH  - Humans
MH  - Lentivirus/*genetics
MH  - Plasmids
MH  - *Transduction, Genetic
EDAT- 2004/02/19 05:00
MHDA- 2004/03/20 05:00
CRDT- 2004/02/19 05:00
PHST- 2004/02/19 05:00 [pubmed]
PHST- 2004/03/20 05:00 [medline]
PHST- 2004/02/19 05:00 [entrez]
AID - 1-59259-650-9:451 [pii]
AID - 10.1385/1-59259-650-9:451 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2004;246:451-9. doi: 10.1385/1-59259-650-9:451.