PMID- 14970701
OWN - NLM
STAT- MEDLINE
DCOM- 20040607
LR  - 20171101
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 102
IP  - 1-4
DP  - 2003
TI  - An ordered BAC contig map of the equine major histocompatibility complex.
PG  - 189-95
AB  - A physical map of ordered bacterial artificial chromosome (BAC) clones was 
      constructed to determine the genetic organization of the horse major 
      histocompatibility complex. Human, cattle, pig, mouse, and rat MHC gene sequences 
      were compared to identify highly conserved regions which served as source 
      templates for the design of overgo primers. Thirty-five overgo probes were 
      designed from 24 genes and used for hybridization screening of the equine USDA 
      CHORI 241 BAC library. Two hundred thirty-eight BAC clones were assembled into 
      two contigs spanning the horse MHC region. The first contig contains the MHC 
      class II region and was reduced to a minimum tiling path of nine BAC clones that 
      span approximately 800 kb and contain at least 20 genes. A minimum tiling path of 
      a second contig containing the class III/I region is comprised of 14 BAC clones 
      that span approximately 1.6 Mb and contain at least 34 genes. Fluorescence in 
      situ hybridization (FISH) using representative clones from each of the three 
      regions of the MHC localized the contigs onto ECA20q21 and oriented the regions 
      relative to one another and the centromere. Dual-colored FISH revealed that the 
      class I region is proximal to the centromere, the class II region is distal, and 
      the class III region is located between class I and II. These data indicate that 
      the equine MHC is a single gene-dense region similar in structure and 
      organization to the human MHC and is not disrupted as in ruminants and pigs.
CI  - Copyright 2003 S. Karger AG, Basel
FAU - Gustafson, A L
AU  - Gustafson AL
AD  - Department of Veterinary Anatomy and Public Health, College of Veterinary 
      Medicine, Texas A&M University, College Station, TX 77843-4458, USA.
FAU - Tallmadge, R L
AU  - Tallmadge RL
FAU - Ramlachan, N
AU  - Ramlachan N
FAU - Miller, D
AU  - Miller D
FAU - Bird, H
AU  - Bird H
FAU - Antczak, D F
AU  - Antczak DF
FAU - Raudsepp, T
AU  - Raudsepp T
FAU - Chowdhary, B P
AU  - Chowdhary BP
FAU - Skow, L C
AU  - Skow LC
LA  - eng
GR  - HD-34086/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Blotting, Southern/methods/veterinary
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Contig Mapping/*methods/*veterinary
MH  - DNA/genetics
MH  - DNA Fingerprinting/methods/veterinary
MH  - Horses/*genetics
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Major Histocompatibility Complex/*genetics
EDAT- 2004/02/19 05:00
MHDA- 2004/06/21 10:00
CRDT- 2004/02/19 05:00
PHST- 2003/05/03 00:00 [received]
PHST- 2003/07/30 00:00 [accepted]
PHST- 2004/02/19 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/02/19 05:00 [entrez]
AID - 75747 [pii]
AID - 10.1159/000075747 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2003;102(1-4):189-95. doi: 10.1159/000075747.