PMID- 1497091
OWN - NLM
STAT- MEDLINE
DCOM- 19920910
LR  - 20220316
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 141
IP  - 2
DP  - 1992 Aug
TI  - Cytokine-induced neutrophil-derived interleukin-8.
PG  - 397-407
AB  - During acute inflammation, the first line of cellular response for host defense 
      is the neutrophil. In addition to the historic role of the neutrophil as a 
      phagocyte, recent studies have identified this cell as an important source of a 
      number of cytokines. In this study, we provide evidence that the neutrophil is a 
      significant source of interleukin-8 (IL-8). Neutrophils freshly isolated from 
      whole blood were not found to constitutively express IL-8 mRNA. In contrast, when 
      these leukocytes were cultured on plastic they were activated, leading to the 
      significant expression of de novo steady-state levels of IL-8 mRNA. In addition, 
      when neutrophils were treated with cycloheximide, there was evidence for 
      "superinduction" of steady-state levels of IL-8 mRNA and inhibition of antigenic 
      IL-8 production. Neutrophils were subsequently stimulated with lipopolysaccharide 
      (LPS), tumor necrosis factor-alpha, or interleukin-1-beta and were found to 
      express IL-8 mRNA and antigen in both a time- and dose-dependent manner. 
      Furthermore, neutrophils stimulated with traditional chemotactic/activating 
      factors, such as the split product of the fifth component of complement (C5a), 
      formylmethionyleucylphenylalanine (fMLP), and leukotriene B4 (LTB4) in a 
      dose-dependent manner did not produce significant antigenic IL-8, as compared 
      with unstimulated controls. In contrast, when neutrophils were exposed to either 
      of these neutrophil agonists in the presence of LPS, the production of antigenic 
      IL-8 was significantly elevated, as compared with either of the stimuli alone, 
      suggesting a synergistic response. These data would suggest that the neutrophil 
      can no longer be viewed as only a phagocyte or warehouse for proteolytic enzymes, 
      but is a pivotal effector cell that is able to respond to mediators in its 
      environment and generate cytokines. This latter neutrophil response may be 
      important for either the elicitation of additional neutrophils or to orchestrate 
      the conventional immune response at sites of inflammation.
FAU - Strieter, R M
AU  - Strieter RM
AD  - Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 
      48109-0360.
FAU - Kasahara, K
AU  - Kasahara K
FAU - Allen, R M
AU  - Allen RM
FAU - Standiford, T J
AU  - Standiford TJ
FAU - Rolfe, M W
AU  - Rolfe MW
FAU - Becker, F S
AU  - Becker FS
FAU - Chensue, S W
AU  - Chensue SW
FAU - Kunkel, S L
AU  - Kunkel SL
LA  - eng
GR  - HL 02401/HL/NHLBI NIH HHS/United States
GR  - HL 31693/HL/NHLBI NIH HHS/United States
GR  - IP50 HL 46487/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Antigens)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plastics)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Antigens/analysis
MH  - Cell Adhesion
MH  - Cytokines/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-1/pharmacology
MH  - Interleukin-8/genetics/immunology/*metabolism
MH  - Lipopolysaccharides
MH  - Neutrophils/*metabolism/physiology
MH  - Plastics
MH  - RNA, Messenger/metabolism
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/pharmacology
PMC - PMC1886610
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
PMCR- 1993/02/01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
PHST- 1993/02/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1992 Aug;141(2):397-407.