PMID- 14971048
OWN - NLM
STAT- MEDLINE
DCOM- 20040316
LR  - 20061115
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 34
IP  - 1
DP  - 2004 Jan
TI  - Complement C1q regulates LPS-induced cytokine production in bone marrow-derived 
      dendritic cells.
PG  - 221-30
AB  - We show here that C1q suppresses IL-12p40 production in LPS-stimulated murine 
      bone marrow-derived dendritic cells (BMDC). Serum IL-12p40 concentration of 
      C1q-deficient mice was higher than that of wild-type mice after intraperitoneal 
      LPS-injection. Because neither globular head of C1q (gC1q) nor collagen-like 
      region of C1q (cC1q) failed to suppress LPS-induced IL-12p40 production, both 
      gC1q and cC1q, and/or some specialized conformation of native C1q may be required 
      for the inhibition. While C1q did not affect mRNA expression of Toll-like 
      receptor 4 (TLR4), MD-2, and myeloid differentiation factor 88 (MyD88), BMDC 
      treated with C1q showed the reduced activity of NF-kappaB and the delayed 
      phosphorylation of p38, c-Jun N-terminal kinase, and extracellular 
      signal-regulated kinase after LPS-stimulation. CpG oligodeoxynucleotide-induced 
      IL-12p40 and TNF-alpha production, another MyD88-dependent TLR-mediated signal, 
      was also suppressed by C1q treatment. Therefore, C1q is likely to suppress 
      MyD88-dependent pathway in TLR-mediated signals. In contrast, C1q failed to 
      suppress colony formation of B cells responding to LPS or LPS-induced CD40 and 
      CD86 expression on BMDC in MyD88-deficient mice, indicating that inhibitory 
      effects of C1q on MyD88-independent pathways may be limited. Taken together, C1q 
      may regulate innate and adaptive immune systems via modification of signals 
      mediated by interactions between invading pathogens and TLR.
FAU - Yamada, Masahide
AU  - Yamada M
AD  - Department of Internal Medicine and Molecular Science, Graduate School of 
      Medicine, Osaka University, Osaka, Japan.
FAU - Oritani, Kenji
AU  - Oritani K
FAU - Kaisho, Tsuneyasu
AU  - Kaisho T
FAU - Ishikawa, Jun
AU  - Ishikawa J
FAU - Yoshida, Hitoshi
AU  - Yoshida H
FAU - Takahashi, Isao
AU  - Takahashi I
FAU - Kawamoto, Shinichirou
AU  - Kawamoto S
FAU - Ishida, Naoko
AU  - Ishida N
FAU - Ujiie, Hidetoshi
AU  - Ujiie H
FAU - Masaie, Hiroaki
AU  - Masaie H
FAU - Botto, Marina
AU  - Botto M
FAU - Tomiyama, Yoshiaki
AU  - Tomiyama Y
FAU - Matsuzawa, Yuji
AU  - Matsuzawa Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Antigens, Ly)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Ly96 protein, mouse)
RN  - 0 (Lymphocyte Antigen 96)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Protein Subunits)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
RN  - 187348-17-0 (Interleukin-12)
RN  - 80295-33-6 (Complement C1q)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Antigens, Differentiation/genetics/metabolism
MH  - Antigens, Ly/genetics/metabolism
MH  - Bone Marrow Cells/*metabolism
MH  - Complement C1q/*metabolism
MH  - Cytokines/*biosynthesis
MH  - Dendritic Cells/*metabolism
MH  - Female
MH  - Interleukin-12/biosynthesis
MH  - Interleukin-12 Subunit p40
MH  - Lipopolysaccharides/metabolism
MH  - Lymphocyte Antigen 96
MH  - Membrane Glycoproteins/genetics/metabolism
MH  - Mice
MH  - Myeloid Differentiation Factor 88
MH  - Protein Subunits/biosynthesis
MH  - RNA, Messenger/metabolism
MH  - Receptors, Cell Surface/genetics/metabolism
MH  - Receptors, Immunologic/genetics/metabolism
MH  - Signal Transduction/physiology
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
EDAT- 2004/02/19 05:00
MHDA- 2004/03/18 05:00
CRDT- 2004/02/19 05:00
PHST- 2004/02/19 05:00 [pubmed]
PHST- 2004/03/18 05:00 [medline]
PHST- 2004/02/19 05:00 [entrez]
AID - 10.1002/eji.200324026 [doi]
PST - ppublish
SO  - Eur J Immunol. 2004 Jan;34(1):221-30. doi: 10.1002/eji.200324026.