PMID- 14972448
OWN - NLM
STAT- MEDLINE
DCOM- 20040928
LR  - 20181130
IS  - 0143-4004 (Print)
IS  - 0143-4004 (Linking)
VI  - 25
IP  - 2-3
DP  - 2004 Feb-Mar
TI  - A primary cell culture system for human cytotrophoblasts of proximal 
      cytotrophoblast cell columns enabling in vitro acquisition of the extra-villous 
      phenotype.
PG  - 153-65
AB  - Cytotrophoblast (CT) differentiation into the extra-villous phenotype is a 
      crucial process in initiating their invasion into the decidua and thereby 
      developing the placenta. However, how CTs differentiate into extra-villous CTs 
      (EVCTs) is not fully elucidated. To address this, a suitable culture model for 
      CTs has been long-sought. But this has been hampered by annoying problems such 
      as; cell aggregation, in vitro syncytialization, low plating efficiency, etc. The 
      aim of this study is to develop a culture system in which CTs differentiate into 
      EVCTs. CTs were isolated from the first trimester placenta using density gradient 
      separation and immuno-depletion using anti-CD9 antibody to remove contaminating 
      fibroblasts and EVCTs. The resultant isolated CTs were found to have the 
      character similar to poorly differentiated CTs comprising proximal 
      cytotrophoblastic cell columns as confirmed by immunocytochemical and 
      flowcytometric analyses. When cultured on type 4 collagen-coated plates in 
      culture media containing low calcium concentration, CTs neither aggregated nor 
      syncytialized, remaining mononuclear and monolayer state. Interestingly, cultured 
      CTs gradually upregulated integrin alpha1, CD9, and human leukocyte antigen 
      (HLA)-G; the known markers specific for EVCTs invading into the decidua 
      diffusely. Hence, the CT culture system provides a sophisticated experimental 
      model in which highly purified CTs acquire the extra-villous phenotype without 
      syncytialization.
FAU - Nagamatsu, T
AU  - Nagamatsu T
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, University of 
      Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
FAU - Fujii, T
AU  - Fujii T
FAU - Ishikawa, T
AU  - Ishikawa T
FAU - Kanai, T
AU  - Kanai T
FAU - Hyodo, H
AU  - Hyodo H
FAU - Yamashita, T
AU  - Yamashita T
FAU - Osuga, Y
AU  - Osuga Y
FAU - Momoeda, M
AU  - Momoeda M
FAU - Kozuma, S
AU  - Kozuma S
FAU - Taketani, Y
AU  - Taketani Y
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Antigens, CD)
RN  - 0 (CD9 protein, human)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Integrin alpha1)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Tetraspanin 29)
SB  - IM
MH  - Adult
MH  - Antigens, CD/metabolism
MH  - Cell Culture Techniques/*methods
MH  - *Cell Differentiation
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Chorion/*cytology
MH  - Female
MH  - HLA Antigens/metabolism
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Integrin alpha1/metabolism
MH  - Membrane Glycoproteins/metabolism
MH  - Models, Biological
MH  - Phenotype
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - Tetraspanin 29
MH  - Trophoblasts/*cytology/metabolism
MH  - Up-Regulation
EDAT- 2004/02/20 05:00
MHDA- 2004/09/29 05:00
CRDT- 2004/02/20 05:00
PHST- 2003/05/19 00:00 [received]
PHST- 2003/08/26 00:00 [revised]
PHST- 2003/08/28 00:00 [accepted]
PHST- 2004/02/20 05:00 [pubmed]
PHST- 2004/09/29 05:00 [medline]
PHST- 2004/02/20 05:00 [entrez]
AID - S0143400403002443 [pii]
AID - 10.1016/j.placenta.2003.08.015 [doi]
PST - ppublish
SO  - Placenta. 2004 Feb-Mar;25(2-3):153-65. doi: 10.1016/j.placenta.2003.08.015.