PMID- 14976466
OWN - NLM
STAT- MEDLINE
DCOM- 20040513
LR  - 20061115
IS  - 0026-4806 (Print)
IS  - 0026-4806 (Linking)
VI  - 94
IP  - 6
DP  - 2003 Dec
TI  - [Cytofluorimetric evaluation of peripheral blood dendritic cells in patients with 
      Mediterranean Kaposi's sarcoma].
PG  - 379-86
AB  - AIM: Kaposi's sarcoma (KS) is a lympho-angioproliferative disorder characterized 
      by angiomatous nodules and plaques that mainly affect the skin. The disease is 
      consistently associated with human herpesvirus-8 (HHV8) and with a state of 
      preexistent immunosuppression. Dendritic cells (DCs) have an instrumental role in 
      the activation and function of both innate and adaptative immune responses. At 
      least 2 distinct subsets have been characterized in peripheral blood based on 
      phenotypic markers: myeloid DCs (CD11c+), associated with Ag uptake, T cell 
      activation and ability to secrete IL-12, and plasmacytoid DCs, high virus-induced 
      IFN-alpha producing cells. Because of the role of both DC subtypes in antiviral 
      and antitumor induced responses, we hypothesized that DCs could be involved in 
      the onset and evolution of KS. METHODS: Thirty-five patients with mediterranean 
      KS assigned to different clinical stages were compared with 51 healthy control 
      subjects. Peripheral blood DCs were quantified and functionally characterised by 
      flow cytometry directly on whole blood samples. The production of the regulatory 
      cytokines, IL-12 and IL-10, was assessed as intracellular accumulation after 
      incubation with or without lipopolysaccharide (LPS). RESULTS: Myeloid DCs 
      identified as lineage-/HLA-DR+/CD11c+ cells were significantly lower in KS 
      patients than in controls (0.54+/-0.25 vs 0.69 +/-0.26% of the peripheral blood 
      mononuclear cells; p<0.017). Furthermore, CD11c+ DCs were lower in patients with 
      more diffuse disease. Plasmacytoid DCs, identified as lineage-/HLA-DR+/CD123+ 
      cells, were lower in KS patients (0.23+/-0.19 vs 0.36+/-0.17; p<0.001). DCs from 
      KS patients were more mature, as assessed by expression of the maturation marker 
      CD83, and showed an impaired ability to produce IL-12 upon LPS stimulation, as 
      compared with controls. CONCLUSION: The numerical and functional alterations of 
      peripheral blood DCs observed in KS patients suggest an involvement of these 
      cells in the onset and evolution of the disease.
FAU - Vaccari, M
AU  - Vaccari M
AD  - Cattedra di Immunologia, Dipartimento Scienze e Tecnologie Biomediche, Universita 
      degli Studi di Milano, Milan, Italy.
FAU - Della Bella, S
AU  - Della Bella S
FAU - Brambilla, L
AU  - Brambilla L
FAU - Ferrucci, S
AU  - Ferrucci S
FAU - Nicola, S
AU  - Nicola S
FAU - Berti, E
AU  - Berti E
FAU - Boneschi, V
AU  - Boneschi V
FAU - Villa, M L
AU  - Villa ML
LA  - ita
PT  - English Abstract
PT  - Journal Article
TT  - Valutazione citofluorimetrica di cellule dendritiche circolanti in pazienti 
      affetti da sarcoma di Kaposi mediterraneo.
PL  - Italy
TA  - Minerva Med
JT  - Minerva medica
JID - 0400732
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Aged
MH  - Case-Control Studies
MH  - Dendritic Cells/*cytology/immunology/metabolism
MH  - Female
MH  - Flow Cytometry/methods
MH  - Fluorescent Antibody Technique, Direct
MH  - Humans
MH  - Immunity, Cellular
MH  - Immunophenotyping
MH  - Interleukin-10/blood
MH  - Interleukin-12/blood
MH  - Male
MH  - Neoplasm Staging
MH  - Sarcoma, Kaposi/*blood/immunology/pathology
EDAT- 2004/02/21 05:00
MHDA- 2004/05/14 05:00
CRDT- 2004/02/21 05:00
PHST- 2004/02/21 05:00 [pubmed]
PHST- 2004/05/14 05:00 [medline]
PHST- 2004/02/21 05:00 [entrez]
PST - ppublish
SO  - Minerva Med. 2003 Dec;94(6):379-86.