PMID- 14977729
OWN - NLM
STAT- MEDLINE
DCOM- 20040315
LR  - 20081121
IS  - 0006-3185 (Print)
IS  - 0006-3185 (Linking)
VI  - 206
IP  - 1
DP  - 2004 Feb
TI  - Differences in the rDNA-bearing chromosome divide the Asian-Pacific and Atlantic 
      species of Crassostrea (Bivalvia, Mollusca).
PG  - 46-54
AB  - Karyotype and chromosomal location of the major ribosomal RNA genes (rDNA) were 
      studied using fluorescence in situ hybridization (FISH) in five species of 
      CRASSOSTREA: three Asian-Pacific species (C. gigas, C. plicatula, and C. 
      ariakensis) and two Atlantic species (C. virginica and C. rhizophorae). FISH 
      probes were made by PCR amplification of the intergenic transcribed spacer 
      between the 18S and 5.8S rRNA genes, and labeled with digoxigenin-11-dUTP. All 
      five species had a haploid number of 10 chromosomes. The Atlantic species had 1-2 
      submetacentric chromosomes, while the three Pacific species had none. FISH with 
      metaphase chromosomes detected a single telomeric locus for rDNA in all five 
      species without any variation. In all three Pacific species, rDNA was located on 
      the long arm of Chromosome 10 (10q)--the smallest chromosome. In the two Atlantic 
      species, rDNA was located on the short arm of Chromosome 2 (2p)--the second 
      longest chromosome. A review of other studies reveals the same distribution of 
      NOR sites (putative rDNA loci) in three other species: on 10q in C. sikamea and 
      C. angulata from the Pacific Ocean and on 2p in C. gasar from the western 
      Atlantic. All data support the conclusion that differences in size and shape of 
      the rDNA-bearing chromosome represent a major divide between Asian-Pacific and 
      Atlantic species of CRASSOSTREA: This finding suggests that chromosomal 
      divergence can occur under seemingly conserved karyotypes and may play a role in 
      reproductive isolation and speciation.
FAU - Wang, Yongping
AU  - Wang Y
AD  - Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, 
      Rutgers University, 6959 Miller Avenue, Port Norris, New Jersey 08349, USA.
FAU - Xu, Zhe
AU  - Xu Z
FAU - Guo, Ximing
AU  - Guo X
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biol Bull
JT  - The Biological bulletin
JID - 2984727R
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Atlantic Ocean
MH  - *Chromosome Mapping
MH  - Chromosomes/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - *Genetic Variation
MH  - Geography
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Ostreidae/*genetics
MH  - Pacific Ocean
EDAT- 2004/02/24 05:00
MHDA- 2004/03/17 05:00
CRDT- 2004/02/24 05:00
PHST- 2004/02/24 05:00 [pubmed]
PHST- 2004/03/17 05:00 [medline]
PHST- 2004/02/24 05:00 [entrez]
AID - 10.2307/1543197 [doi]
PST - ppublish
SO  - Biol Bull. 2004 Feb;206(1):46-54. doi: 10.2307/1543197.