PMID- 14978753
OWN - NLM
STAT- MEDLINE
DCOM- 20041007
LR  - 20121115
IS  - 1099-498X (Print)
IS  - 1099-498X (Linking)
VI  - 6 Suppl 1
DP  - 2004 Feb
TI  - Surface-engineering of lentiviral vectors.
PG  - S83-94
AB  - Vectors derived from retroviridae offer particularly flexible properties in gene 
      transfer applications given the numerous possible associations of various viral 
      surface glycoproteins (determining cell tropism) with different types of 
      retroviral cores (determining genome replication and integration). Lentiviral 
      vectors should be preferred gene delivery vehicles over vectors derived from 
      onco-retroviruses such as murine leukemia viruses (MLVs) that cannot transduce 
      non-proliferating target cells. Generating lentiviral vectors pseudotyped with 
      different viral glycoproteins (GPs) may modulate the physicochemical properties 
      of the vectors, their interaction with the host immune system and their host 
      range. There are however important gene transfer restrictions to some 
      non-proliferative tissues or cell types and recent studies have shown that 
      progenitor hematopoietic stem cells in G(0), non-activated primary blood 
      lymphocytes or monocytes were not transducible by lentiviral vectors. Moreover, 
      lentiviral vectors that have the capacity to deliver transgenes into specific 
      tissues are expected to be of great value for various gene transfer applications 
      in vivo. Several innovative approaches have been explored to overcome such 
      problems that have given rise to novel concepts in the field and have provided 
      promising results in preliminary evaluations in vivo. Here we review the 
      different approaches explored to upgrade lentiviral vectors, aiming at developing 
      vectors suitable for in vivo gene delivery.
CI  - Copyright 2004 John Wiley & Sons, Ltd.
FAU - Verhoeyen, Els
AU  - Verhoeyen E
AD  - Laboratoire de Vectorologie Retrovirale et Therapie Genique, INSERM U412, Ecole 
      Normale Superieure de Lyon, IFR128 BioSciences Lyon-Gerland. Lyon, France.
FAU - Cosset, Francois-Loic
AU  - Cosset FL
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - J Gene Med
JT  - The journal of gene medicine
JID - 9815764
RN  - 0 (Ligands)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - *Genetic Engineering
MH  - Genetic Therapy
MH  - Genetic Vectors/*chemistry
MH  - Humans
MH  - Lentivirus/*chemistry
MH  - Ligands
MH  - Recombinant Fusion Proteins/chemistry
MH  - Viral Envelope Proteins/*chemistry
RF  - 141
EDAT- 2004/02/24 05:00
MHDA- 2004/10/08 09:00
CRDT- 2004/02/24 05:00
PHST- 2004/02/24 05:00 [pubmed]
PHST- 2004/10/08 09:00 [medline]
PHST- 2004/02/24 05:00 [entrez]
AID - 10.1002/jgm.494 [doi]
PST - ppublish
SO  - J Gene Med. 2004 Feb;6 Suppl 1:S83-94. doi: 10.1002/jgm.494.