PMID- 14981687
OWN - NLM
STAT- MEDLINE
DCOM- 20041015
LR  - 20121115
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 25
IP  - 4-5
DP  - 2004 Feb
TI  - Determination of basic pharmaceuticals in human serum by hydrophilic interaction 
      capillary electrochromatography.
PG  - 600-6
AB  - Hydrophilic interaction capillary electrochromatography (HI-CEC) for the 
      determination of basic pharmaceuticals spiked in human serum is described. The 
      organic modifier content, ionic strength, and pH value of the mobile phase as 
      well as the applied voltage are optimized for separation and elution of these 
      drug analytes. Excellent separation was achieved for drugs using a mobile phase 
      composition of 80% v/v acetonitrile in 100 mM triethylamine phosphate (TEAP) 
      buffer at pH 2.8 with column efficiencies for analytes more than 200,000 
      plates/m. The samples of human serum spiked with basic drugs were directly 
      injected after a simple acetonitrile treatment. The linear range and 
      reproducibility of these basic drugs using an external and internal standard 
      method were compared. As a result, the reproducibility could be greatly improved 
      by using the internal standard method. Good calibration curves with regression 
      coefficients more than 0.998 in the range of 5-160 microg/mL were observed with 
      the internal standard method. The limits of quantitation, based on standards with 
      acceptable relative standard deviations (RSDs), were below 5 microg/mL. The 
      intra- and inter-day precisions, determined as RSDs, were less than 4.57%.
FAU - Fu, Hongjing
AU  - Fu H
AD  - National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The 
      Chinese Academy of Sciences, Dalian, China.
FAU - Jin, Wenhai
AU  - Jin W
FAU - Xiao, Hua
AU  - Xiao H
FAU - Xie, Chuanhui
AU  - Xie C
FAU - Guo, Baochuan
AU  - Guo B
FAU - Zou, Hanfa
AU  - Zou H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Acetonitriles)
RN  - 0 (Buffers)
RN  - 0 (Ethylamines)
RN  - 0 (Pharmaceutical Preparations)
RN  - VOU728O6AY (triethylamine)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetonitriles/chemistry
MH  - Buffers
MH  - Calibration
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Drug Interactions
MH  - Ethylamines/chemistry
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Pharmaceutical Preparations/*blood/chemistry
MH  - Reference Standards
MH  - Reproducibility of Results
EDAT- 2004/02/26 05:00
MHDA- 2004/10/16 09:00
CRDT- 2004/02/26 05:00
PHST- 2004/02/26 05:00 [pubmed]
PHST- 2004/10/16 09:00 [medline]
PHST- 2004/02/26 05:00 [entrez]
AID - 10.1002/elps.200305757 [doi]
PST - ppublish
SO  - Electrophoresis. 2004 Feb;25(4-5):600-6. doi: 10.1002/elps.200305757.