PMID- 14982516
OWN - NLM
STAT- MEDLINE
DCOM- 20050210
LR  - 20161124
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 59
IP  - 3
DP  - 2004 Mar
TI  - Isolation and characterization of a heat-resistant beef allergen: myoglobin.
PG  - 327-31
AB  - BACKGROUND: Meat allergy is rarely reported. Most of the described cases are 
      sensitizations to bovine serum albumin. OBJECTIVE: The aim of the study was to 
      describe a case of allergy to a new meat allergen and, after its 
      characterization. METHODS: A 35-year-old nonatopic female with allergic episodes 
      after ingestion of several types of meat was studied. Skin tests (prick and 
      prick-to-prick); total and specific immunoglobulin E (IgE) determination; sodium 
      dodecyl sulphate-polyacrylamide gel electrophoresis and specific IgE 
      determination by immunoblotting under different conditions were performed. A 
      17-kDa allergen was semipurified by ethanol fractionation and its amino-terminal 
      sequence was determined. The existence of specific IgE directed to this protein 
      was studied by immunoblot in 80 atopic patients. RESULTS: The patient showed 
      specific IgE antibodies to a 17-kDa protein. During the isolation of this 
      allergen it was found that a 70-90% (vol/vol) ethanol concentration was able to 
      purify the protein. The characterization revealed that it was a heat-resistant 
      protein without disulfide bonds. N-terminal amino acid sequence (16 residues) 
      showed identity with myoglobin. The study of specific IgE to this allergen among 
      atopic patients showed that it was recognized by about 1% of the subjects. 
      CONCLUSIONS: We describe a case of meat allergy caused by myoglobin. This is the 
      first described case of monosensitization to this protein.
FAU - Fuentes, Ma M
AU  - Fuentes MM
AD  - Hospital General Yague, Burgos, Spain.
FAU - Palacios, R
AU  - Palacios R
FAU - Garces, Ma M
AU  - Garces M
FAU - Caballero, M L
AU  - Caballero ML
FAU - Moneo, I
AU  - Moneo I
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Myoglobin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
CIN - Allergy. 2005 Jan;60(1):126. PMID: 15575944
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cattle
MH  - Female
MH  - Food Hypersensitivity/*etiology
MH  - Hot Temperature
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Meat/*adverse effects
MH  - Molecular Sequence Data
MH  - Myoglobin/*immunology
MH  - Skin Tests
EDAT- 2004/02/26 05:00
MHDA- 2005/02/11 09:00
CRDT- 2004/02/26 05:00
PHST- 2004/02/26 05:00 [pubmed]
PHST- 2005/02/11 09:00 [medline]
PHST- 2004/02/26 05:00 [entrez]
AID - ALL266 [pii]
AID - 10.1111/j.1398-9995.2004.00266.x [doi]
PST - ppublish
SO  - Allergy. 2004 Mar;59(3):327-31. doi: 10.1111/j.1398-9995.2004.00266.x.