PMID- 14984098
OWN - NLM
STAT- MEDLINE
DCOM- 20040924
LR  - 20191108
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 12
IP  - 1
DP  - 2004
TI  - Multicolor chromosome painting in diagnostic and research applications.
PG  - 15-23
AB  - For many years whole chromosome painting probes have been the work-horses in a 
      large variety of clinical and research molecular cytogenetic applications. In 
      recent years painting probes have been complemented by an increasing number of 
      further region-specific probes, which allow the specific staining of centromeres, 
      subtelomeres or other regions within the genome. This development of new probe 
      sets was greatly facilitated by the Human Genome Project from which 
      well-characterized probes for any region within the genome have emerged. 
      Furthermore, the evolution of different multicolor fluorescence in situ 
      hybridization (FISH) technologies now allows the cohybridization of multiple 
      DNA-probes of different colors. These developments have paved the way for 
      FISH-based automated karyotyping or the simultaneous analysis of multiple defined 
      regions within the genome. Using appropriate instrumentation and image 
      processing, the analysis can be performed two-dimensionally on metaphase spreads 
      or three-dimensionally in intact interphase nuclei. Here we summarize some of the 
      most recent developments and discuss the application of painting probes in 
      different scenarios.
FAU - Langer, Sabine
AU  - Langer S
AD  - Institut fur Humangenetik, Technische Universitat Munchen, Trogerstr. 32, D-81675 
      Munchen, Germany.
FAU - Kraus, Jurgen
AU  - Kraus J
FAU - Jentsch, Isabell
AU  - Jentsch I
FAU - Speicher, Michael R
AU  - Speicher MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Chromosome Painting/instrumentation/*methods/trends
MH  - DNA Probes/genetics
MH  - Female
MH  - Humans
MH  - Interphase
MH  - Karyotyping
MH  - Metaphase
MH  - Mice
MH  - *Research Design
RF  - 63
EDAT- 2004/02/27 05:00
MHDA- 2004/09/25 05:00
CRDT- 2004/02/27 05:00
PHST- 2004/02/27 05:00 [pubmed]
PHST- 2004/09/25 05:00 [medline]
PHST- 2004/02/27 05:00 [entrez]
AID - 10.1023/b:chro.0000009326.21752.88 [doi]
PST - ppublish
SO  - Chromosome Res. 2004;12(1):15-23. doi: 10.1023/b:chro.0000009326.21752.88.