PMID- 14984368
OWN - NLM
STAT- MEDLINE
DCOM- 20041018
LR  - 20181113
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 380
IP  - Pt 2
DP  - 2004 Jun 1
TI  - Cloning and characterization of the human beta2-glycoprotein I (beta2-GPI) gene 
      promoter: roles of the atypical TATA box and hepatic nuclear factor-1alpha in 
      regulating beta2-GPI promoter activity.
PG  - 455-63
AB  - Beta2-glycoprotein I (beta2-GPI) is a plasma glycoprotein primarily synthesized 
      in the liver. The interindividual variability of beta2-GPI expression in subjects 
      with various metabolic syndromes and disease states suggests that it may have 
      clinical importance. However, the regulation of beta2-GPI gene expression has not 
      been clarified. To gain more insight into the control of beta2-GPI gene 
      expression, we cloned the 4.1-kb 5'-flanking region and characterized the 
      proximal promoter of the beta2- GPI gene in this study. Cis -acting elements 
      required for beta2-GPI promoter activity were identified with transient 
      transfection assays in the hepatoma cell lines HepG2 and Huh7 and in non-hepatic 
      HeLa cells. Serial deletion analyses of the beta2-GPI 5'-flanking sequence 
      revealed that the region from -197 to +7 had strong promoter activity in hepatoma 
      cells but not in HeLa cells. Truncation and site-directed mutagenesis of putative 
      cis -elements within this region showing an atypical TATA box and a HNF-1 
      (hepatic nuclear factor-1) element were both essential for the beta2-GPI promoter 
      activity. Subsequent gel mobility shift assays confirmed the interaction of 
      HNF-1alpha with the HNF-1 site residing downstream of the TATA box. 
      Co-transfection of beta2-GPI promoter-luciferase vector with HNF-1alpha 
      expression vector in Huh7 and HNF-1-deficient HeLa cells demonstrated the 
      transactivation effect of HNF-1alpha on beta2-GPI promoter activity. In addition, 
      overexpression of HNF-1alpha enhanced the endogenous beta2-GPI expression. These 
      results suggest that the atypical TATA box and HNF-1 cis-element are critical for 
      beta2-GPI transcription and HNF-1alpha may play an important role in 
      cell-specific regulation of beta2-GPI gene expression.
FAU - Wang, Hsueh-Hsiao
AU  - Wang HH
AD  - Institute of Biochemistry, School of Life Science, National Yang-Ming University, 
      Taipei 112, Taiwan, ROC.
FAU - Chiang, An-Na
AU  - Chiang AN
LA  - eng
SI  - GENBANK/AY376239
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (HNF1A protein, human)
RN  - 0 (Hepatocyte Nuclear Factor 1-alpha)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (beta 2-Glycoprotein I)
RN  - 126548-29-6 (Hepatocyte Nuclear Factor 1)
SB  - IM
MH  - 5' Flanking Region/genetics
MH  - Base Sequence
MH  - Carcinoma, Hepatocellular/genetics/pathology
MH  - Cell Line, Tumor
MH  - Cloning, Molecular/*methods
MH  - DNA, Neoplasm/genetics/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Electrophoretic Mobility Shift Assay/methods
MH  - Enhancer Elements, Genetic/genetics
MH  - Glycoproteins/*genetics
MH  - HeLa Cells/chemistry/metabolism/pathology
MH  - Hepatocyte Nuclear Factor 1
MH  - Hepatocyte Nuclear Factor 1-alpha
MH  - Humans
MH  - Liver Neoplasms/genetics/pathology
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Promoter Regions, Genetic/*genetics
MH  - TATA Box/*genetics
MH  - Transcription Factors/metabolism
MH  - Transcription Initiation Site
MH  - Transcriptional Activation/genetics
MH  - Transfection/methods
MH  - beta 2-Glycoprotein I
PMC - PMC1224180
EDAT- 2004/02/27 05:00
MHDA- 2004/10/19 09:00
PMCR- 2004/12/01
CRDT- 2004/02/27 05:00
PHST- 2004/02/25 00:00 [accepted]
PHST- 2004/02/19 00:00 [revised]
PHST- 2003/10/22 00:00 [received]
PHST- 2004/02/27 05:00 [pubmed]
PHST- 2004/10/19 09:00 [medline]
PHST- 2004/02/27 05:00 [entrez]
PHST- 2004/12/01 00:00 [pmc-release]
AID - BJ20031610 [pii]
AID - 10.1042/BJ20031610 [doi]
PST - ppublish
SO  - Biochem J. 2004 Jun 1;380(Pt 2):455-63. doi: 10.1042/BJ20031610.