PMID- 14989701
OWN - NLM
STAT- MEDLINE
DCOM- 20040402
LR  - 20191108
IS  - 0959-4493 (Print)
IS  - 0959-4493 (Linking)
VI  - 15
IP  - 1
DP  - 2004 Feb
TI  - In vitro metabolism of dehydroepiandrosterone and testosterone by canine hair 
      follicle cells.
PG  - 19-24
AB  - The metabolism of radioactive dehydroepiandrosterone (DHEA) and testosterone was 
      studied in dermal papilla cells (DPC) and dermal fibroblasts (DFB) derived from 
      hair follicles from two different body sites (head, flank) of four male, 
      castrated beagle dogs. Thin layer chromatography was used for separation, and 
      autoradiography for identification of the radioactive metabolites. DHEA was 
      metabolized mainly to 11 alpha-OH-testosterone and only to a minor extent to 11 
      alpha-OH-androstenedione and another unidentified metabolite. The highest 
      percentage of metabolization of DHEA was found in DFB of the head. Testosterone 
      was metabolized only to a minor extent (less than 10%) to 5 
      alpha-dihydrotestosterone and epiandrosterone and there was no significant 
      difference between either the two cell types or the two locations. These results 
      clearly show that the metabolization of androgens in canine DPC and DFB is 
      different from that observed in cells from the human hair follicle.
FAU - Bamberg, Elmar
AU  - Bamberg E
AD  - Institut fur Biochemie und Ludwig Boltzmann-Institut fur Veterinarmedizinische 
      Endokrinologie, Veterinarmedizinische Universitat Wien, Veterinarplatz 1, A-1210 
      Vienna, Austria. elmar.bamberg@vu-wien.ac.at
FAU - Aichinger, Amanda
AU  - Aichinger A
FAU - Mitteregger, Gerda
AU  - Mitteregger G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Dermatol
JT  - Veterinary dermatology
JID - 9426187
RN  - 3XMK78S47O (Testosterone)
RN  - 459AG36T1B (Dehydroepiandrosterone)
SB  - IM
MH  - Animals
MH  - Dehydroepiandrosterone/*pharmacokinetics
MH  - Dogs/*metabolism
MH  - Fibroblasts/metabolism
MH  - Hair Follicle/cytology/*metabolism
MH  - Male
MH  - Testosterone/*pharmacokinetics
EDAT- 2004/03/03 05:00
MHDA- 2004/04/03 05:00
CRDT- 2004/03/03 05:00
PHST- 2004/03/03 05:00 [pubmed]
PHST- 2004/04/03 05:00 [medline]
PHST- 2004/03/03 05:00 [entrez]
AID - 366 [pii]
AID - 10.1111/j.1365-3164.2004.00366.x [doi]
PST - ppublish
SO  - Vet Dermatol. 2004 Feb;15(1):19-24. doi: 10.1111/j.1365-3164.2004.00366.x.