PMID- 14990232
OWN - NLM
STAT- MEDLINE
DCOM- 20040506
LR  - 20090602
IS  - 1074-7427 (Print)
IS  - 1074-7427 (Linking)
VI  - 81
IP  - 2
DP  - 2004 Mar
TI  - Spatial learning in rats is impaired by microinfusions of protein kinase C-gamma 
      antisense oligodeoxynucleotide within the nucleus accumbens.
PG  - 120-36
AB  - The nucleus accumbens (NAcc) has been shown to play a role in motor and spatial 
      learning. Protein kinase C (PKC) has been implicated in the mechanisms of 
      initiation and maintenance of long-term potentiation that is thought to be 
      involved in the storage of long-term memory. In the present study, the importance 
      of de novo synthesis of PKC-gamma within the NAcc in the acquisition and 
      retention of spatial discrimination learning was assessed using an antisense 
      knockdown approach. Separate groups of Long-Evans rats were exposed to acute 
      microinfusions (6microg/microl) of PKC-gamma antisense oligodeoxynucleotide 
      (AS-ODN), control oligodeoxynucleotide (C-ODN) or vehicle into the NAcc at 24 and 
      3h before each training session. Behavioral findings showed that the blockade of 
      NAcc-PKC-gamma translation caused impairments in the early phase of learning and 
      retention of spatial information. Biochemical experiments showed that PKC-gamma 
      expression was reduced and Ca(2+)/phospholipid-dependent protein kinase C (PKC) 
      activity was blocked significantly in the AS-ODN-treated rats in comparison with 
      control rats. The present findings suggest that NAcc-PKC-gamma plays a role 
      during the early acquisition of spatial learning. Also, retention test results 
      suggest that NAcc-PKC-gamma may be working as an intermediate factor involved in 
      the onset of molecular mechanisms necessary for spatial memory consolidation 
      within the NAcc.
FAU - Alvarez-Jaimes, Lily
AU  - Alvarez-Jaimes L
AD  - Department of Biology, University of Puerto Rico, Rio Piedras Campus, P.O. Box 
      23360, San Juan 00931-3360, Puerto Rico.
FAU - Betancourt, Elba
AU  - Betancourt E
FAU - Centeno-Gonzalez, Marjorie
AU  - Centeno-Gonzalez M
FAU - Feliciano-Rivera, Mara Z
AU  - Feliciano-Rivera MZ
FAU - Rodriguez, Danella
AU  - Rodriguez D
FAU - Pena de Ortiz, Sandra
AU  - Pena de Ortiz S
FAU - Maldonado-Vlaar, Carmen S
AU  - Maldonado-Vlaar CS
LA  - eng
GR  - 1 GM5S06GM08103/GM/NIGMS NIH HHS/United States
GR  - 1 P20 RR15565 02/RR/NCRR NIH HHS/United States
GR  - 1 R29 DA11665-04/DA/NIDA NIH HHS/United States
GR  - R25 GM6-1151-01/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neurobiol Learn Mem
JT  - Neurobiology of learning and memory
JID - 9508166
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - EC 2.7.1.- (protein kinase C gamma)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Animals
MH  - Cognition Disorders/chemically induced
MH  - Immunoblotting
MH  - Learning/*drug effects
MH  - Male
MH  - Microinjections
MH  - Nucleus Accumbens/*drug effects
MH  - Oligodeoxyribonucleotides, Antisense/administration & dosage/adverse 
      effects/*pharmacology
MH  - Protein Kinase C/administration & dosage/adverse effects/*pharmacology
MH  - Rats
MH  - Rats, Long-Evans
MH  - Space Perception/*drug effects
EDAT- 2004/03/03 05:00
MHDA- 2004/05/07 05:00
CRDT- 2004/03/03 05:00
PHST- 2003/07/12 00:00 [received]
PHST- 2003/12/03 00:00 [revised]
PHST- 2003/12/05 00:00 [accepted]
PHST- 2004/03/03 05:00 [pubmed]
PHST- 2004/05/07 05:00 [medline]
PHST- 2004/03/03 05:00 [entrez]
AID - S1074742703001278 [pii]
AID - 10.1016/j.nlm.2003.12.001 [doi]
PST - ppublish
SO  - Neurobiol Learn Mem. 2004 Mar;81(2):120-36. doi: 10.1016/j.nlm.2003.12.001.