PMID- 14990342
OWN - NLM
STAT- MEDLINE
DCOM- 20040423
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1688
IP  - 2
DP  - 2004 Mar 2
TI  - Modulation of rat erythrocyte antioxidant defense system by buthionine 
      sulfoximine and its reversal by glutathione monoester therapy.
PG  - 121-9
AB  - The protective effects of glutathione monoester (GME) on buthionine sulfoximine 
      (BSO)-induced glutathione (GSH) depletion and its sequel were evaluated in rat 
      erythrocyte/erythrocyte membrane. Animals were divided into three groups (n=6 in 
      each): control, BSO and BSO+GME group. Administration of BSO, at a concentration 
      of 4 mmol/kg bw, to the albino rats resulted in depletion of blood GSH level to 
      about 59%. GSH was elevated several folds in the GME group as compared to the 
      control (P<0.05) and BSO (P<0.001) groups. Decreased concentration of vitamin E 
      was found in the erythrocyte membrane isolated from BSO-administered animals. 
      Antioxidant enzymes, catalase (CAT), superoxide dismutase (SOD) and glutathione 
      peroxidase (GPX) were also found to be altered due to BSO-induced GSH depletion 
      in blood erythrocytes. The SOD and CAT activities in BSO group were significantly 
      lower (P<0.001) than the other groups. Lipid peroxidation index and 
      malondialdehyde (MDA) levels in erythrocytes and their membranes were increased 
      to about 45% and 40%, respectively. The activities of Ca2+ ATPase, Mg2+ ATPase 
      and Na+K+ ATPase were lower than those of control group (P<0.05), whereas the 
      activities of these enzymes were found to be restored to normal followed by GME 
      therapy (P<0.05). Cholesterol, phospholipid and C/P ratio and some of the 
      phospholipid classes like phosphatidylcholine (PC), lysophosphatidylcholine (LPC) 
      and sphingomyelin were significantly (P<0.05) altered in the erythrocyte 
      membranes of BSO-administered rats compared with those of control group. These 
      parameters were restored to control group levels in GME-treated group. Oxidative 
      stress may play a major role in the BSO-mediated gamma glutamyl cysteine 
      synthetase (gamma-GCS) inhibition and hence the depletion of GSH. In conclusion, 
      our findings have shown that antioxidant status decreased and lipid peroxidation 
      increased in BSO-treated rats. GME potentiates the RBC and blood antioxidant 
      defense mechanisms and decreases lipid peroxidation.
FAU - Rajasekaran, Namakkal Surappan
AU  - Rajasekaran NS
AD  - Unit of Biochemistry, Department of Zoology, University of Madras, Guindy Campus, 
      Chennai, 600 025, India. nsrajachem@rediffmail.com
FAU - Devaraj, Niranjali S
AU  - Devaraj NS
FAU - Devaraj, Halagowder
AU  - Devaraj H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Antioxidants)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Membrane Lipids)
RN  - 0 (Phospholipids)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 1406-18-4 (Vitamin E)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 5072-26-4 (Buthionine Sulfoximine)
RN  - 7W95D60F4J (S-ethyl glutathione)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Adenosine Triphosphatases/blood
MH  - Animals
MH  - Antioxidants/analysis/*metabolism
MH  - Buthionine Sulfoximine/administration & dosage/antagonists & 
      inhibitors/*pharmacology
MH  - Catalase/blood
MH  - Chromatography, High Pressure Liquid
MH  - Enzyme Inhibitors/pharmacology
MH  - Erythrocyte Membrane/drug effects/metabolism
MH  - Erythrocytes/*drug effects/metabolism
MH  - Glutathione/*analogs & derivatives/deficiency/*therapeutic use
MH  - Glutathione Peroxidase/blood
MH  - Hydrogen Peroxide
MH  - Lipid Peroxidation
MH  - Male
MH  - Malondialdehyde/blood
MH  - Membrane Lipids/blood
MH  - Oxidative Stress/drug effects
MH  - Phospholipids/blood
MH  - Rats
MH  - Rats, Wistar
MH  - Superoxide Dismutase/blood
MH  - Thiobarbituric Acid Reactive Substances/analysis
MH  - Vitamin E/blood
EDAT- 2004/03/03 05:00
MHDA- 2004/04/24 05:00
CRDT- 2004/03/03 05:00
PHST- 2003/09/09 00:00 [received]
PHST- 2003/11/07 00:00 [revised]
PHST- 2003/11/11 00:00 [accepted]
PHST- 2004/03/03 05:00 [pubmed]
PHST- 2004/04/24 05:00 [medline]
PHST- 2004/03/03 05:00 [entrez]
AID - S0925443903001911 [pii]
AID - 10.1016/j.bbadis.2003.11.004 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2004 Mar 2;1688(2):121-9. doi: 
      10.1016/j.bbadis.2003.11.004.