PMID- 14990852
OWN - NLM
STAT- MEDLINE
DCOM- 20041112
LR  - 20061115
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 22
IP  - 2
DP  - 2004
TI  - Enhancement of allogeneic hematopoietic stem cell engraftment and prevention of 
      GVHD by intra-bone marrow bone marrow transplantation plus donor lymphocyte 
      infusion.
PG  - 125-34
AB  - We examined the effect of intra-bone marrow (IBM)-bone marrow transplantation 
      (BMT) in conjunction with donor lymphocyte infusion (DLI) on the engraftment of 
      allogeneic bone marrow cells (BMCs) in mice. Recipients that had received 6 Gy of 
      radiation completely rejected donor BMCs, even when IBM-BMT was carried out. 
      However, when BMCs were IBM injected and donor peripheral blood mononuclear cells 
      (PBMNCs) were simultaneously injected intravenously (DLI), donor cell engraftment 
      was observed 7 days after BMT and complete donor chimerism continued thereafter. 
      It is of interest that the cells of recipient origin did not recover, and that 
      the hematolymphoid cells, including progenitor cells (Lin-/c-kit+ cells) in the 
      recipients, were fully reconstituted with cells of donor origin. The cells in the 
      PBMNCs responsible for the donor BMC engraftment were CD8+. Recipients that had 
      received 6 Gy of radiation, IBM-BMT, and DLI showed only a slight loss of body 
      weight, due to radiation side effects, and had no macroscopic or microscopic 
      symptoms of graft-versus-host disease. These findings suggest that IBM-BMT in 
      conjunction with DLI will be a valuable strategy for allogeneic BMT in humans.
FAU - Nakamura, Koichi
AU  - Nakamura K
AD  - First Department of Pathology, Kansai Medical University, Osaka, Japan.
FAU - Inaba, Muneo
AU  - Inaba M
FAU - Sugiura, Kikuya
AU  - Sugiura K
FAU - Yoshimura, Tomoo
AU  - Yoshimura T
FAU - Kwon, A-Hon
AU  - Kwon AH
FAU - Kamiyama, Yasuo
AU  - Kamiyama Y
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
SB  - IM
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cell Transplantation/methods
MH  - *Lymphocyte Transfusion
MH  - Lymphocytes/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Radiation
EDAT- 2004/03/03 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/03/03 05:00
PHST- 2004/03/03 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/03/03 05:00 [entrez]
AID - 10.1634/stemcells.22-2-125 [doi]
PST - ppublish
SO  - Stem Cells. 2004;22(2):125-34. doi: 10.1634/stemcells.22-2-125.