PMID- 14991720
OWN - NLM
STAT- MEDLINE
DCOM- 20041006
LR  - 20071114
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 229
IP  - 3
DP  - 2004 Mar
TI  - FISHing for chick genes: Triple-label whole-mount fluorescence in situ 
      hybridization detects simultaneous and overlapping gene expression in avian 
      embryos.
PG  - 651-7
AB  - Multi-color whole-mount in situ hybridization is a powerful technique for 
      comparing the spatial expression patterns of two or more genes in developing 
      embryos. We have developed an amplified triple-label whole-mount fluorescence in 
      situ hybridization (FISH) protocol that permits detection of three different 
      mRNAs in a single embryo. Our protocol uses simultaneous in situ hybridization to 
      haptenylated riboprobes, followed by sequential antibody detection using 
      anti-hapten antibodies conjugated to horseradish peroxidase, and the tyramide 
      signal amplification (TSA) fluorescence detection system. Conventional 
      fluorescence microscopy identifies areas of overlapping gene expression at the 
      tissue level, whereas confocal fluorescence microscopy permits single-cell 
      resolution and differentiates specialized cell types within a given tissue. This 
      protocol will provide researchers engaged in the use of FISH with a solid 
      starting point for adapting their own in situ hybridization protocols, either 
      alone or in combination with immunohistochemistry or green fluorescence protein 
      colocalization.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Denkers, Nathaniel
AU  - Denkers N
AD  - Pediatric Nephrology, University of Utah School of Medicine, Salt Lake City, Utah 
      84132-2204, USA.
FAU - Garcia-Villalba, Pilar
AU  - Garcia-Villalba P
FAU - Rodesch, Christopher K
AU  - Rodesch CK
FAU - Nielson, Kandice R
AU  - Nielson KR
FAU - Mauch, Teri Jo
AU  - Mauch TJ
LA  - eng
GR  - 02490/PHS HHS/United States
GR  - 62864/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of 
      Anatomists
JID - 9201927
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (PAX2 Transcription Factor)
RN  - 0 (Transcription Factors)
RN  - 93586-27-7 (chordin)
SB  - IM
MH  - Animals
MH  - Chick Embryo
MH  - DNA-Binding Proteins/metabolism
MH  - Developmental Biology/*methods
MH  - *Gene Expression Regulation, Developmental
MH  - Glycoproteins/metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Mesoderm/metabolism
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence/methods
MH  - PAX2 Transcription Factor
MH  - Time Factors
MH  - Transcription Factors/metabolism
EDAT- 2004/03/03 05:00
MHDA- 2004/10/07 09:00
CRDT- 2004/03/03 05:00
PHST- 2004/03/03 05:00 [pubmed]
PHST- 2004/10/07 09:00 [medline]
PHST- 2004/03/03 05:00 [entrez]
AID - 10.1002/dvdy.20005 [doi]
PST - ppublish
SO  - Dev Dyn. 2004 Mar;229(3):651-7. doi: 10.1002/dvdy.20005.