PMID- 14991773
OWN - NLM
STAT- MEDLINE
DCOM- 20041210
LR  - 20131121
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 91
IP  - 4
DP  - 2004 Mar 1
TI  - Activity-dependent gene regulation in conditionally-immortalized muscle precursor 
      cell lines.
PG  - 821-39
AB  - Skeletal muscle contractile activity has been implicated in many aspects of 
      muscle cell differentiation and maturation. Much of the research in this area has 
      depended upon costly and labor-intensive cultures of isolated primary muscle 
      cells because widely available immortalized muscle cell lines often do not 
      display a high level of either spontaneous or stimulated contractile activity. We 
      sought to develop conditionally-immortalized skeletal muscle cell lines that 
      would provide a source of myofibers that exhibit robust spontaneous contractile 
      activity similar to primary muscle cultures. Using a tetracycline-regulated 
      retroviral vector expressing a temperature-sensitive T-antigen to infect primary 
      myoblasts, we isolated individual clonal muscle precursor cell lines that have 
      characteristics of activated satellite cells during growth and rapidly 
      differentiate into mature myotubes with spontaneous contractile activity after 
      culture in non-transformation-permissive conditions. Comparison of these cell 
      lines (known as rat myoblast-like tetracycline (RMT) cell lines) to primary cell 
      cultures revealed that they share a wide variety of morphological, physiological, 
      and biochemical characteristics. Most importantly, the time-course and extent of 
      activity-dependent gene regulation observed in primary cell culture for all genes 
      tested, including subunits of the nicotinic acetylcholine receptor (nAChR), 
      muscle specific kinase (MuSK), and myogenin, is reproduced in RMT lines. These 
      immortalized cell lines are a useful alternative to primary cultures for studying 
      muscle differentiation and molecular and physiological aspects of electrical 
      activity in muscle fibers.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Macpherson, Peter C D
AU  - Macpherson PC
AD  - Mental Health Research Institute, University of Michigan, Ann Arbor, 205 Zina 
      Pitcher Pl., Ann Arbor, Michigan 48109-0720, USA. petercdm@umich.edu
FAU - Suhr, Steven T
AU  - Suhr ST
FAU - Goldman, Daniel
AU  - Goldman D
LA  - eng
GR  - 5R01WS025153-16/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Antigens, Polyomavirus Transforming)
RN  - 0 (Cdkn1a protein, rat)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (MyoD Protein)
RN  - 0 (Myog protein, rat)
RN  - 0 (Myogenic Regulatory Factor 5)
RN  - 0 (Myogenic Regulatory Factors)
RN  - 0 (Myogenin)
RN  - 0 (PAX7 Transcription Factor)
RN  - 0 (Receptors, Cholinergic)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Trans-Activators)
RN  - 0 (myogenic factor 6)
RN  - F8VB5M810T (Tetracycline)
SB  - IM
MH  - Animals
MH  - Antigens, Polyomavirus Transforming/genetics/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Line, Transformed
MH  - Cells, Cultured
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclins/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Electric Stimulation
MH  - Gene Expression Regulation/*drug effects
MH  - Homeodomain Proteins/metabolism
MH  - Immunohistochemistry
MH  - Muscle Contraction
MH  - Muscle Proteins/metabolism
MH  - Muscle, Skeletal/*cytology/*drug effects/metabolism/virology
MH  - MyoD Protein/metabolism
MH  - Myogenic Regulatory Factor 5
MH  - Myogenic Regulatory Factors/metabolism
MH  - Myogenin/metabolism
MH  - PAX7 Transcription Factor
MH  - Rats
MH  - Receptors, Cholinergic/metabolism
MH  - Retinoblastoma Protein/metabolism
MH  - Retroviridae/genetics
MH  - Simian virus 40/genetics
MH  - Stem Cells/cytology/*drug effects/metabolism/virology
MH  - Temperature
MH  - Tetracycline/*pharmacology
MH  - Trans-Activators/metabolism
EDAT- 2004/03/03 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/03/03 05:00
PHST- 2004/03/03 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/03/03 05:00 [entrez]
AID - 10.1002/jcb.10784 [doi]
PST - ppublish
SO  - J Cell Biochem. 2004 Mar 1;91(4):821-39. doi: 10.1002/jcb.10784.