PMID- 14991934
OWN - NLM
STAT- MEDLINE
DCOM- 20040412
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 10
IP  - 5
DP  - 2004 Mar 1
TI  - Inhibitor RNA blocks the protein translation mediated by hepatitis C virus 
      internal ribosome entry site in vivo.
PG  - 664-7
AB  - AIM: To investigate the inhibitory effect of hepatitis C virus internal ribosome 
      entry site (HCV IRES) specific inhibitor RNA (IRNA) on gene expression mediated 
      by HCV IRES in vivo. METHODS: By using G418 screening system, hepatoma cells 
      constitutively expressing IRNA or mutant IRNA (mIRNA) were established and 
      characterized, and HCV replicons containing the 5' untranslated region (5'UTR) 
      were constructed by using the same method. Cotransfection of pCMVNCRluc 
      containing HCV 5'UTR-luc fusion genes and eukaryotic vector of IRNA into human 
      hepatic carcinoma cells (HepG2) was performed and the eukaryotic expression 
      plasmid of IRNA was transfected transiently into HCV replicons. pCMVNCRluc or 
      pCDNA-luc was cotransfected with pSV40-beta Gal into IRNA expressing hepatoma 
      cells by using lipofectamine 2000 in vitro. Then the reporting gene expression 
      level was examined at 48 h after transfection by using a luminometer and the 
      expressing level of HCV C antigen was analysed with a confocal microscope. 
      RESULTS: Transient expression of IRES specific IRNA could significantly inhibit 
      the expression of reporter gene and viral antigen mediated by HCV IRES by 50% to 
      90% in vivo, but mIRNA lost its inhibitory activity completely. The luciferase 
      gene expression mediated by HCV IRES was blocked in the HHCC constitutively 
      expressing IRNA. At 48 h after transfection, the expression level of reporter 
      gene decreased by 20%, but cap-dependent luciferase gene expression was not 
      affected. IRNA could inhibit the HCV replicon expression 24 h after transfection 
      and the highest inhibitory activity was 80% by 72 h, and the inhibitory activity 
      was not increased until 7d after transfection. CONCLUSION: IRNA can inhibit HCV 
      IRES mediated gene expression in vivo.
FAU - Liang, Xue-Song
AU  - Liang XS
AD  - Department of Infectious Diseases, Changhai Hospital, Second Military Medical 
      University, Shanghai, China. liangxuesong2000@163.com
FAU - Lian, Jian-Qi
AU  - Lian JQ
FAU - Zhou, Yong-Xing
AU  - Zhou YX
FAU - Wan, Mo-Bin
AU  - Wan MB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Carcinoma, Hepatocellular
MH  - Gene Expression Regulation, Viral
MH  - Hepacivirus/*genetics
MH  - Hepatitis C/*virology
MH  - Humans
MH  - Liver Neoplasms
MH  - *Protein Biosynthesis
MH  - RNA, Viral
MH  - Replicon/genetics
MH  - Ribosomes/*genetics/*virology
MH  - Transfection
PMC - PMC4716905
EDAT- 2004/03/03 05:00
MHDA- 2004/04/13 05:00
PMCR- 2004/03/01
CRDT- 2004/03/03 05:00
PHST- 2004/03/03 05:00 [pubmed]
PHST- 2004/04/13 05:00 [medline]
PHST- 2004/03/03 05:00 [entrez]
PHST- 2004/03/01 00:00 [pmc-release]
AID - 10.3748/wjg.v10.i5.664 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2004 Mar 1;10(5):664-7. doi: 10.3748/wjg.v10.i5.664.