PMID- 14994175
OWN - NLM
STAT- MEDLINE
DCOM- 20041109
LR  - 20181113
IS  - 0095-3628 (Print)
IS  - 0095-3628 (Linking)
VI  - 47
IP  - 3
DP  - 2004 Apr
TI  - Sulfate-reducing bacteria-dominated biofilms that precipitate ZnS in a subsurface 
      circumneutral-pH mine drainage system.
PG  - 205-17
AB  - The microbial diversity of ZnS-forming biofilms in 8 degrees C, circumneutral-pH 
      groundwater in tunnels within the abandoned Piquette Zn, Pb mine (Tennyson, 
      Wisconsin, USA) has been investigated by molecular methods, fluorescence in situ 
      hybridization (FISH), and cultivation techniques. These biofilms are growing on 
      old mine timbers that generate locally anaerobic zones within the mine drainage 
      system. Sulfate-reducing bacteria (SRB) exclusively of the family 
      Desulfobacteriaceae comprise a significant fraction of the active microbiota. 
      Desulfosporosinus strains were isolated, but could not be detected by molecular 
      methods. Other important microbial clusters belonged to the beta-, gamma-, and 
      epsilon-Proteobacteria, the Cytophaga/Flexibacter/Bacteroides-group (CFB), 
      Planctomycetales, Spirochaetales, Clostridia, and green nonsulfur bacteria. Our 
      investigations indicated a growth dependence of SRB on fermentative, 
      cellulolytic, and organic acid-producing Clostridia. A few clones related to 
      sulfur-oxidizing bacteria were detected, suggesting a sulfur cycle related to 
      redox gradients within the biofilm. Sulfur oxidation prevents sulfide 
      accumulation that would lead to precipitation of other sulfide phases. FISH 
      analyses indicated that Desulfobacteriaceae populations were not early colonizers 
      in freshly grown and ZnS-poor biofilms, whereas they were abundant in older, 
      naturally established, and ZnS-rich biofilms. Gram-negative SRB have been 
      detected in situ over a period of 6 months, supporting the important role of 
      these organisms in selective ZnS precipitation in Tennyson mine. Results 
      demonstrate the complex nature of biofilms responsible for in situ bioremediation 
      of toxic metals in a subsurface mine drainage system.
FAU - Labrenz, M
AU  - Labrenz M
AD  - Department of Geology and Geophysics, University of Wisconsin-Madison, 1215 W. 
      Dayton St., Madison, WI 53706, USA. matthias.labrenz@io-warnemuende.de
FAU - Banfield, J F
AU  - Banfield JF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20040304
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sulfides)
RN  - 0 (Zinc Compounds)
RN  - KPS085631O (zinc sulfide)
SB  - IM
MH  - Bacteria/genetics
MH  - Base Sequence
MH  - Biodegradation, Environmental
MH  - *Biofilms
MH  - Clostridium/metabolism
MH  - Cluster Analysis
MH  - DNA Primers
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Electron
MH  - *Mining
MH  - Molecular Sequence Data
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Species Specificity
MH  - Sulfides/*metabolism
MH  - Sulfur-Reducing Bacteria/growth & development/*metabolism/ultrastructure
MH  - Symbiosis
MH  - *Water Microbiology
MH  - Water Purification/methods
MH  - Wisconsin
MH  - Zinc Compounds/*metabolism
EDAT- 2004/03/03 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/03/03 05:00
PHST- 2003/02/06 00:00 [received]
PHST- 2003/06/17 00:00 [accepted]
PHST- 2004/03/03 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/03/03 05:00 [entrez]
AID - 10.1007/s00248-003-1025-8 [doi]
PST - ppublish
SO  - Microb Ecol. 2004 Apr;47(3):205-17. doi: 10.1007/s00248-003-1025-8. Epub 2004 Mar 
      4.