PMID- 14998290
OWN - NLM
STAT- MEDLINE
DCOM- 20040317
LR  - 20081121
IS  - 1233-9687 (Print)
IS  - 1233-9687 (Linking)
VI  - 54
IP  - 4
DP  - 2003
TI  - PCR analysis of matrix metalloproteinase 3 (MMP-3) gene promoter polymorphism in 
      ovarian cancer.
PG  - 233-8
AB  - Matrix metalloproteinases (MMPs) are proteolytic enzymes capable of degrading 
      extracellular matrix. Their role has been emphasized in tumor invasion, 
      metastasis and tumor-induced angiogenesis. The gene encoding MMP-3 is polymorphic 
      and an insertion (6A)/deletion (5A) polymorphism (5A/6A polymorphism) in the 
      MMP-3 gene may have functional significance in the regulation of its expression. 
      In the present work the distribution of genotypes and frequency of alleles of the 
      5A/6A polymorphism in subjects with ovarian cancer were investigated. Paraffin 
      embedded tumor tissues were obtained from 118 postmenopausal women with 
      node-negative and node-positive ovarian cancer. The 5A/6A polymorphism was 
      determined by PCR amplification using the allele specific primers. The 
      distribution of the genotypes of the 5A/6A polymorphism in both control and study 
      patients did not differ significantly (p>0.05) from those predicted by the 
      Hardy-Weinberg distribution. There were no significant differences (p>0.05) in 
      genotype distributions and allele frequencies between subgroups assigned to 
      histological stage. The results suggest that the 5A/6A polymorphism of MMP-3 gene 
      may not be linked with appearance and/or progression of ovarian cancer.
FAU - Smolarz, Beata
AU  - Smolarz B
AD  - Laboratory of Molecular Genetics, Department of Pathology, Institute of Polish 
      Mother's Memorial Hospital, Lodz.
FAU - Szyllo, Krzysztof
AU  - Szyllo K
FAU - Romanowicz-Makowska, Hanna
AU  - Romanowicz-Makowska H
FAU - Niewiadomski, Mariusz
AU  - Niewiadomski M
FAU - Kozlowska, Elzbieta
AU  - Kozlowska E
FAU - Kulig, Andrzej
AU  - Kulig A
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Pol J Pathol
JT  - Polish journal of pathology : official journal of the Polish Society of 
      Pathologists
JID - 9437432
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Neoplasm)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adenocarcinoma/enzymology/*genetics/secondary
MH  - Aged
MH  - Aged, 80 and over
MH  - DNA Primers/chemistry
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Gene Frequency
MH  - Humans
MH  - Loss of Heterozygosity
MH  - Matrix Metalloproteinase 3/*genetics
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Ovarian Neoplasms/enzymology/*genetics/pathology
MH  - Polymerase Chain Reaction/*methods
MH  - *Polymorphism, Genetic
MH  - Postmenopause
MH  - Promoter Regions, Genetic
EDAT- 2004/03/05 05:00
MHDA- 2004/03/18 05:00
CRDT- 2004/03/05 05:00
PHST- 2004/03/05 05:00 [pubmed]
PHST- 2004/03/18 05:00 [medline]
PHST- 2004/03/05 05:00 [entrez]
PST - ppublish
SO  - Pol J Pathol. 2003;54(4):233-8.