PMID- 14998822
OWN - NLM
STAT- MEDLINE
DCOM- 20040628
LR  - 20190513
IS  - 0735-0414 (Print)
IS  - 0735-0414 (Linking)
VI  - 39
IP  - 2
DP  - 2004 Mar-Apr
TI  - Chronic ethanol consumption regulates cannabinoid CB1 receptor gene expression in 
      selected regions of rat brain.
PG  - 88-92
AB  - AIMS: The aim of this study was to examine the effects of chronic ethanol 
      consumption in cannabinoid CB(1) receptor gene expression in Wistar rats. 
      METHODS: Rats were exposed to a bottle containing a solution of ethanol (10% v/v) 
      and saccharin (0.25% w/v) for 52 days. At the end of this period, rats were 
      killed by decapitation and cannabinoid CB(1) receptor gene expression was 
      measured by in situ hybridization histochemistry. RESULTS: Our results indicated 
      that chronic ethanol consumption reduced cannabinoid CB(1) receptor gene 
      expression in caudate-putamen (CPu) (24%), ventromedial nucleus of the 
      hypothalamus (VMN) (43%), CA1 (27%) and CA2 (22%) fields of hippocampus and 
      increased dentate gyrus (DG) (30%). CONCLUSIONS: These results reveal for the 
      first time that prolonged exposure to ethanol produces marked alterations in 
      cannabinoid CB(1) receptor gene expression in selected regions of the rat brain, 
      supporting an interaction between ethanol consumption and the endogenous 
      cannabinoid receptor. Furthermore, these findings suggest that cannabinoid CB(1) 
      receptor may be considered as a new pharmacological target for treating ethanol 
      dependence.
FAU - Ortiz, Sergio
AU  - Ortiz S
AD  - Servicio de Psiquiatria y Unidad de Investigacion, Hospital Universitario 12 de 
      Octubre, Madrid, Spain.
FAU - Oliva, Jose M
AU  - Oliva JM
FAU - Perez-Rial, Sandra
AU  - Perez-Rial S
FAU - Palomo, Tomas
AU  - Palomo T
FAU - Manzanares, Jorge
AU  - Manzanares J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Alcohol Alcohol
JT  - Alcohol and alcoholism (Oxford, Oxfordshire)
JID - 8310684
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Cannabinoid, CB1)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Alcohol Drinking/*metabolism
MH  - Animals
MH  - Brain/drug effects/*metabolism
MH  - Ethanol/*administration & dosage
MH  - Gene Expression Regulation/drug effects/physiology
MH  - Male
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Cannabinoid, CB1/*biosynthesis/genetics
EDAT- 2004/03/05 05:00
MHDA- 2004/06/29 05:00
CRDT- 2004/03/05 05:00
PHST- 2004/03/05 05:00 [pubmed]
PHST- 2004/06/29 05:00 [medline]
PHST- 2004/03/05 05:00 [entrez]
AID - 10.1093/alcalc/agh036 [doi]
PST - ppublish
SO  - Alcohol Alcohol. 2004 Mar-Apr;39(2):88-92. doi: 10.1093/alcalc/agh036.