PMID- 14999802
OWN - NLM
STAT- MEDLINE
DCOM- 20040729
LR  - 20181130
IS  - 1043-3074 (Print)
IS  - 1043-3074 (Linking)
VI  - 26
IP  - 3
DP  - 2004 Mar
TI  - Genetically targeted radiotherapy of head and neck squamous cell carcinoma using 
      the sodium-iodide symporter (NIS).
PG  - 265-71
AB  - BACKGROUND: Gene therapy that uses delivery of the sodium-iodide symporter (NIS) 
      gene followed by radioiodide administration has been proposed as a novel form of 
      radiotherapy for nonthyroidal cancers. METHODS: In vitro [(125)I] iodide 
      accumulation and efflux from cells was determined after treatment with an 
      NIS-expressing adenovirus (Ad-NIS). A clonogenic survival assay and tumor growth 
      experiment that used athymic mice were used to demonstrate the in vitro and in 
      vivo cytotoxicity of Ad-NIS treatment and [(131)I] iodide delivery. RESULTS: Head 
      and neck squamous cell carcinoma (HNSCC) cell lines treated with Ad-NIS exhibit 
      significant amounts of radioiodide accumulation and retention. In vitro HNSCC 
      cell survival was significantly diminished after NIS gene delivery followed by 
      administration of [(131)I] iodide. Moreover, NIS gene transfer/[(131)I] iodide 
      administration dramatically attenuated HNSCC tumor formation in athymic mice. 
      CONCLUSIONS: Our data demonstrate the feasibility of genetically targeted 
      radiotherapy by use of the NIS gene as a possible therapeutic intervention in 
      head and neck cancer.
CI  - Copyright 2004 Wiley Periodicals, Inc. Head Neck 26: 265-271, 2004
FAU - Gaut, Andrew W
AU  - Gaut AW
AD  - Department of Otolaryngology-Head and Neck Surgery, Roy J. and Lucille A. Carver 
      College of Medicine, University of Iowa, Iowa City, Iowa, USA.
FAU - Niu, Gang
AU  - Niu G
FAU - Krager, Kimberly J
AU  - Krager KJ
FAU - Graham, Michael M
AU  - Graham MM
FAU - Trask, Douglas K
AU  - Trask DK
FAU - Domann, Frederick E
AU  - Domann FE
LA  - eng
GR  - CA91709/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Head Neck
JT  - Head & neck
JID - 8902541
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Symporters)
RN  - 4XE5NDT4K1 (sodium-iodide symporter)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*radiotherapy
MH  - Cell Line, Tumor
MH  - Feasibility Studies
MH  - Female
MH  - Genetic Therapy/*methods
MH  - Head and Neck Neoplasms/genetics/metabolism/*radiotherapy
MH  - Iodine Radioisotopes/administration & dosage
MH  - Mice
MH  - Mice, Nude
MH  - Symporters/*genetics/metabolism
EDAT- 2004/03/05 05:00
MHDA- 2004/07/30 05:00
CRDT- 2004/03/05 05:00
PHST- 2004/03/05 05:00 [pubmed]
PHST- 2004/07/30 05:00 [medline]
PHST- 2004/03/05 05:00 [entrez]
AID - 10.1002/hed.10369 [doi]
PST - ppublish
SO  - Head Neck. 2004 Mar;26(3):265-71. doi: 10.1002/hed.10369.