PMID- 15001429
OWN - NLM
STAT- MEDLINE
DCOM- 20040723
LR  - 20131121
IS  - 0193-1857 (Print)
IS  - 0193-1857 (Linking)
VI  - 287
IP  - 1
DP  - 2004 Jul
TI  - Vitamin E attenuates biochemical and morphological features associated with 
      development of chronic pancreatitis.
PG  - G162-9
AB  - The objective was to investigate the effects of vitamin E on collagen deposition 
      induced by Cyclosporin A (CsA) administration in rats with caerulein (Cr) 
      pancreatitis. CsA transforms the fully regenerative, self-limited form of Cr 
      pancreatitis into a chroniclike disease in conjunction with increased 
      transforming growth factor (TGF)-beta and myofibroblast proliferation. Vitamin E 
      inhibits TGF-beta release in mesangial cells and reduces CsA cytotoxicity. Wistar 
      rats received CsA daily (20 mg/kg), and CR pancreatitis was induced on days 1 and 
      8 (Cr + CsA group). In a separate group, vitamin E (600 mg.kg(-1).day(-1)) was 
      administered starting 4 days before CsA. Three other groups received either 
      vehicle, CsA, or Cr alone. Thiobarbituric acid-reactive substance (TBARS), 
      8-isoprostanes, and hyaluronic acid were measured in plasma obtained on the day 
      the animals were killed (day 15). Pancreases were weighed and processed for light 
      microscopy to assess connective tissue and myofibroblast number. Pancreatic 
      homogenates were also assayed for collagen (hydroxyproline) and TBARS content. 
      TBARS, 8-isoprostane, and TGF-beta were elevated in CsA and Cr + CsA rats. 
      Vitamin E treatment greatly decreased these parameters. Vitamin E also decreased 
      the fall in pancreatic weight observed in Cr + CsA pancreas. Pancreatic 
      hydroxyproline and plasma hyaluronic acid were increased in Cr + CsA rats but 
      were effectively reduced by vitamin E. Morphology showed improvement in fibrosis 
      score and a decreased number of myofibroblasts in vitamin E-treated rats. Vitamin 
      E reduces oxidative stress and collagen deposition during the development of 
      experimental chronic pancreatitis. Adjuvant antioxidants may be of value in the 
      treatment of chronic pancreatitis.
FAU - Gomez, Jose-Antonio
AU  - Gomez JA
AD  - Servei d'Aparell Digestiu, Hospital Universitari Vall d'Hebron, Passeig Vall 
      d'Hebron 119-129, 08035 Barcelona, Spain.
FAU - Molero, Xavier
AU  - Molero X
FAU - Vaquero, Eva
AU  - Vaquero E
FAU - Alonso, Ana
AU  - Alonso A
FAU - Salas, Antonio
AU  - Salas A
FAU - Malagelada, Juan-R
AU  - Malagelada JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040304
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (Lipoproteins)
RN  - 1406-18-4 (Vitamin E)
RN  - 83HN0GTJ6D (Cyclosporine)
RN  - 888Y08971B (Ceruletide)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Ceruletide
MH  - Chronic Disease
MH  - Collagen/metabolism
MH  - Cyclosporine/pharmacology
MH  - Fibroblasts/pathology
MH  - Fibrosis
MH  - Lipid Peroxidation/drug effects
MH  - Lipoproteins/blood
MH  - Male
MH  - Myocytes, Smooth Muscle/pathology
MH  - Pancreas/drug effects/metabolism/pathology
MH  - Pancreatitis/chemically induced/*metabolism/*pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Vitamin E/blood/*pharmacology
EDAT- 2004/03/06 05:00
MHDA- 2004/07/24 05:00
CRDT- 2004/03/06 05:00
PHST- 2004/03/06 05:00 [pubmed]
PHST- 2004/07/24 05:00 [medline]
PHST- 2004/03/06 05:00 [entrez]
AID - 00333.2003 [pii]
AID - 10.1152/ajpgi.00333.2003 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2004 Jul;287(1):G162-9. doi: 
      10.1152/ajpgi.00333.2003. Epub 2004 Mar 4.