PMID- 15003528
OWN - NLM
STAT- MEDLINE
DCOM- 20040408
LR  - 20100120
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 316
IP  - 1
DP  - 2004 Mar 26
TI  - NF-kappaB binds to a polymorphic repressor element in the MMP-3 promoter.
PG  - 182-8
AB  - A 5T/6T polymorphic site in the matrix metalloproteinase-3 (MMP-3) promoter has 
      been identified as a repressor element involved in inhibiting induction of MMP-3 
      transcription by interleukin 1; and the 6T allele has been associated with 
      decreased expression of MMP-3 as compared to the 5T allele. Zinc-binding 
      protein-89 (ZBP-89) was cloned from a yeast one-hybrid assay via its ability to 
      interact with this site, but when the protein was over-expressed, it resulted in 
      activation of the MMP-3 promoter rather than repression. Here we show that in 
      nuclear extracts isolated from human gingival fibroblasts stimulated with IL-1, 
      this site is bound by p50 and p65 components of NF-kappaB in addition to ZBP-89, 
      and that recombinant p50 binds preferentially to the 6T binding site. These 
      results are consistent with a role for NF-kappaB in limiting the cytokine induced 
      expression of MMP-3.
FAU - Borghaei, Ruth C
AU  - Borghaei RC
AD  - Department of Biochemistry and Molecular Biology, Philadelphia College of 
      Osteopathic Medicine, 4170 City Avenue, Philadelphia, PA 19131, USA. 
      ruthb@pcom.edu
FAU - Rawlings, P Lyle Jr
AU  - Rawlings PL Jr
FAU - Javadi, Masoud
AU  - Javadi M
FAU - Woloshin, Joanna
AU  - Woloshin J
LA  - eng
GR  - R29DE12096/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interleukin-1)
RN  - 0 (NF-kappa B)
RN  - 0 (NF-kappa B p50 Subunit)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ZNF148 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Binding Sites
MH  - DNA-Binding Proteins/metabolism
MH  - Enzyme Repression
MH  - Fibroblasts/drug effects/metabolism
MH  - Gingiva/cytology
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Matrix Metalloproteinase 3/*genetics
MH  - NF-kappa B/*metabolism
MH  - NF-kappa B p50 Subunit
MH  - Polymorphism, Genetic
MH  - *Promoter Regions, Genetic
MH  - Repressor Proteins/*metabolism
MH  - Response Elements
MH  - Transcription Factors/metabolism
EDAT- 2004/03/09 05:00
MHDA- 2004/04/09 05:00
CRDT- 2004/03/09 05:00
PHST- 2004/01/29 00:00 [received]
PHST- 2004/03/09 05:00 [pubmed]
PHST- 2004/04/09 05:00 [medline]
PHST- 2004/03/09 05:00 [entrez]
AID - S0006291X0400275X [pii]
AID - 10.1016/j.bbrc.2004.02.030 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2004 Mar 26;316(1):182-8. doi: 
      10.1016/j.bbrc.2004.02.030.