PMID- 15004463
OWN - NLM
STAT- MEDLINE
DCOM- 20040528
LR  - 20191210
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 103
IP  - 1-2
DP  - 2003
TI  - Multi-locus (ML)-FISH is a reliable tool for nondisjunction studies in human 
      oocytes.
PG  - 47-53
AB  - In the present study, we developed a fluorescence in situ hybridization (FISH) 
      strategy, which allows a reliable determination of the chromatid number of 
      specific chromosomes in mature human oocytes. 168 unfertilized oocytes were 
      analyzed by dual-color FISH with two direct-labeled locus-specific DNA probes for 
      chromosome 13 and 21. To exclude FISH failures, metaphases with abnormal signal 
      patterns were reanalyzed by multi-locus-FISH (ML-FISH) for chromosome 13 and 21. 
      Following dual-color FISH, abnormal signal patterns were detected in 21 out of 
      108 metaphases (19.4%). 17 of these metaphases were reanalyzed by ML-FISH. In 
      contrast to the first FISH, seven metaphases showed normal signal patterns after 
      rehybridization, whereas ten metaphases remained abnormal. Out of these real 
      aneuploid metaphases, five showed gain or loss of a single signal (= chromatid), 
      two showed missing double signals (= chromosome) and three showed both. In 
      conclusion, locus-specific FISH probes facilitate differentiation between first 
      meiotic nondisjunction of whole chromosomes and prematurely divided chromatids. 
      Moreover, simultaneous hybridization with a second locus-specific probe on the 
      same chromatid (ML-FISH) helps to differentiate between FISH failures and real 
      meiotic division errors and therefore, allows a more reliable analysis of 
      aneuploidies in human oocytes.
CI  - Copyright 2003 S. Karger AG, Basel
FAU - Eckel, H
AU  - Eckel H
AD  - Clinic for Reproductive Medicine and Gynecological Endocrinology, Magdeburg, 
      Germany. Heike.Eckel@Medizin.Uni-Magdeburg.de
FAU - Kleinstein, J
AU  - Kleinstein J
FAU - Wieacker, P
AU  - Wieacker P
FAU - Stumm, M
AU  - Stumm M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - Chromatids/ultrastructure
MH  - Chromosomes, Human, Pair 13/ultrastructure
MH  - Chromosomes, Human, Pair 21/ultrastructure
MH  - Color
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Metaphase
MH  - *Nondisjunction, Genetic
MH  - Oocytes/*ultrastructure
EDAT- 2004/03/09 05:00
MHDA- 2004/05/29 05:00
CRDT- 2004/03/09 05:00
PHST- 2003/07/24 00:00 [received]
PHST- 2003/10/27 00:00 [accepted]
PHST- 2004/03/09 05:00 [pubmed]
PHST- 2004/05/29 05:00 [medline]
PHST- 2004/03/09 05:00 [entrez]
AID - 76287 [pii]
AID - 10.1159/000076287 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2003;103(1-2):47-53. doi: 10.1159/000076287.