PMID- 15004480 OWN - NLM STAT- MEDLINE DCOM- 20040528 LR - 20171101 IS - 1424-859X (Electronic) IS - 1424-8581 (Linking) VI - 103 IP - 1-2 DP - 2003 TI - Comparative cytogenetic analysis on four tree frog species (Anura, Hylidae, Hylinae) from Brazil. PG - 155-62 AB - A comparative cytogenetic analysis was carried out on four Hylinae tree frogs from Brazil ((Aparasphenodon brunoi, Corythomantis greeningi, Osteocephalus langsdorffii, and Scinax fuscovarius) using Giemsa staining, BrdU replication banding, Ag-NOR staining, C-banding, DAPI and CMA(3) fluorochrome staining, and fluorescence in situ hybridization (FISH) with an rDNA probe. All the species share closely similar 2n = 24 karyotypes, almost indistinguishable by standard staining. The technique of BrdU incorporation allowed the identification of each pair of homologs and the establishment of extensive homeology for the great majority of the chromosomes, mainly of A. brunoi, C. greeningi, and O. langsdorffii. Despite highly conserved replication banding patterns, the use of the other banding techniques disclosed some minor differences, which reinforces the importance of extensive cytogenetic analyses for the karyotypic characterization of Anuran species. The present cytogenetic data confirm the closer proximity of A. brunoi, C. greeningi, and O. langsdorffii, whereas S. fuscovarius is phylogenetically more distant. CI - Copyright 2003 S. Karger AG, Basel FAU - Kasahara, S AU - Kasahara S AD - Departamento de Biologia, Instituto de Biociencias, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brasil. kasahara@rc.unesp.br FAU - Zampieri Silva, A P AU - Zampieri Silva AP FAU - Gruber, S L AU - Gruber SL FAU - Haddad, C F B AU - Haddad CF LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Cytogenet Genome Res JT - Cytogenetic and genome research JID - 101142708 SB - IM MH - Animals MH - Anura/classification/*genetics MH - Brazil MH - Chromosome Banding MH - *Chromosomes/ultrastructure MH - Cytogenetic Analysis MH - Female MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - Male MH - Nucleolus Organizer Region MH - Synteny EDAT- 2004/03/09 05:00 MHDA- 2004/05/29 05:00 CRDT- 2004/03/09 05:00 PHST- 2003/06/13 00:00 [received] PHST- 2003/08/21 00:00 [accepted] PHST- 2004/03/09 05:00 [pubmed] PHST- 2004/05/29 05:00 [medline] PHST- 2004/03/09 05:00 [entrez] AID - 76304 [pii] AID - 10.1159/000076304 [doi] PST - ppublish SO - Cytogenet Genome Res. 2003;103(1-2):155-62. doi: 10.1159/000076304.