PMID- 15005448
OWN - NLM
STAT- MEDLINE
DCOM- 20040329
LR  - 20191210
IS  - 0042-4900 (Print)
IS  - 0042-4900 (Linking)
VI  - 154
IP  - 8
DP  - 2004 Feb 21
TI  - Evaluation of the role of mallard ducks as vectors of porcine reproductive and 
      respiratory syndrome virus.
PG  - 233-7
AB  - To assess the transmission of porcine reproductive and respiratory syndrome virus 
      (PRRSV) from pigs to mallard ducks, 10 adult (one-year-old) female mallard ducks 
      were housed with pigs infected experimentally with PRRSV, and allowed to be in 
      close contact with them for 21 days. To evaluate the transmission of PRRSV from 
      mallard ducks to pigs, two adult ducks were inoculated orally with PRRSV (total 
      dose 10(6.0) TCID50) and allowed to drink PRRsv-infected water; 24 hours later, 
      two four-week-old PRRsv-naive sentinel pigs were housed in pens below the cages 
      housing the ducks for 14 days. In both experiments, cloacal and faecal samples 
      were collected three times a week from the ducks and tested by PCR, virus 
      isolation and a pig bioassay. Blood samples from the pigs were tested by ELISA, 
      PCR and virus isolation. Sera from the ducks were tested by serum neutralisation. 
      The ducks were examined postmortem and selected tissues were tested by PCR, virus 
      isolation, histopathology and pig bioassay. In both experiments all the cloacal 
      swabs, faecal samples, tissues and sera from the ducks were negative by all the 
      tests. The sera from the pigs in the first experiment were PCR positive at three, 
      seven, 14 and 21 days after infection and ELISA positive at 14 and 21 days. Sera 
      from the pigs in the second experiment were negative by all the tests. The virus 
      was isolated from the oral inoculum and the drinking water provided for the ducks 
      in the second experiment. Under the conditions of this study, it was not possible 
      to demonstrate the transmission of PRRSV either from the pigs to the ducks or 
      from the ducks to the pigs.
FAU - Trincado, C
AU  - Trincado C
AD  - Swine Disease Eradication Center, Room 385C, University of Minnesota College of 
      Veterinary Medicine, 1988 Fitch Avenue, St Paul, MN 55108, USA.
FAU - Dee, S
AU  - Dee S
FAU - Rossow, K
AU  - Rossow K
FAU - Halvorson, D
AU  - Halvorson D
FAU - Pijoan, C
AU  - Pijoan C
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Rec
JT  - The Veterinary record
JID - 0031164
SB  - IM
MH  - Animals
MH  - Cloaca/virology
MH  - *Disease Vectors
MH  - Ducks/*virology
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Feces/virology
MH  - Female
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*transmission
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/*immunology/isolation & purification
MH  - Swine
EDAT- 2004/03/10 05:00
MHDA- 2004/03/30 05:00
CRDT- 2004/03/10 05:00
PHST- 2004/03/10 05:00 [pubmed]
PHST- 2004/03/30 05:00 [medline]
PHST- 2004/03/10 05:00 [entrez]
AID - 10.1136/vr.154.8.233 [doi]
PST - ppublish
SO  - Vet Rec. 2004 Feb 21;154(8):233-7. doi: 10.1136/vr.154.8.233.