PMID- 15006695
OWN - NLM
STAT- MEDLINE
DCOM- 20040601
LR  - 20151119
IS  - 0969-9961 (Print)
IS  - 0969-9961 (Linking)
VI  - 15
IP  - 2
DP  - 2004 Mar
TI  - Sialin expression in the CNS implicates extralysosomal function in neurons.
PG  - 251-61
AB  - SLC17A5 encodes a lysosomal membrane protein, sialin, which transports sialic 
      acid from lysosomes. Mutations in sialin result in neurodegenerative sialic acid 
      storage disorders, Salla disease (SD) and infantile sialic acid storage disease 
      (ISSD). Here we analyzed sialin in mouse central nervous system (CNS) and primary 
      cortical and hippocampal neurons and glia. In the CNS, sialin was predominantly 
      expressed in neurons, especially in the proliferative zone of the prospective 
      neocortex and the hippocampus in developing brain. In nonneuronal cells and 
      primary glial cell cultures, mouse sialin was localized into lysosomes but 
      interestingly, in primary neuronal cultures sialin was not targeted into 
      lysosomes but rather revealed a punctate staining along the neuronal processes 
      and was also seen in the plasma membrane. These data demonstrate a nonlysosomal 
      localization of sialin in neurons and would imply a role for sialin in the 
      secretory processes of neuronal cells.
FAU - Aula, Nina
AU  - Aula N
AD  - Department of Molecular Medicine, National Public Health Institute, Biomedicum, 
      00290 Helsinki, Finland.
FAU - Kopra, Outi
AU  - Kopra O
FAU - Jalanko, Anu
AU  - Jalanko A
FAU - Peltonen, Leena
AU  - Peltonen L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
RN  - 0 (Biomarkers)
RN  - 0 (Organic Anion Transporters)
RN  - 0 (Symporters)
RN  - 0 (sialic acid transport proteins)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Brain/*embryology/*metabolism/ultrastructure
MH  - Cell Differentiation/physiology
MH  - Cell Membrane/*metabolism/ultrastructure
MH  - Cerebral Cortex/cytology/embryology/metabolism
MH  - Fetus
MH  - HeLa Cells
MH  - Hippocampus/embryology/metabolism/ultrastructure
MH  - Humans
MH  - Immunohistochemistry
MH  - Lysosomes/*metabolism
MH  - Mice
MH  - N-Acetylneuraminic Acid/metabolism
MH  - Neurites/metabolism/ultrastructure
MH  - Neuroglia/cytology/metabolism
MH  - Neurons/*metabolism/ultrastructure
MH  - Organic Anion Transporters/*metabolism
MH  - Presynaptic Terminals/metabolism/ultrastructure
MH  - Sialic Acid Storage Disease/genetics/metabolism/pathology/physiopathology
MH  - Symporters/*metabolism
EDAT- 2004/03/10 05:00
MHDA- 2004/06/02 05:00
CRDT- 2004/03/10 05:00
PHST- 2003/06/24 00:00 [received]
PHST- 2003/10/24 00:00 [revised]
PHST- 2003/11/04 00:00 [accepted]
PHST- 2004/03/10 05:00 [pubmed]
PHST- 2004/06/02 05:00 [medline]
PHST- 2004/03/10 05:00 [entrez]
AID - S0969996103002377 [pii]
AID - 10.1016/j.nbd.2003.11.017 [doi]
PST - ppublish
SO  - Neurobiol Dis. 2004 Mar;15(2):251-61. doi: 10.1016/j.nbd.2003.11.017.