PMID- 15007180
OWN - NLM
STAT- MEDLINE
DCOM- 20040713
LR  - 20190911
IS  - 0273-2289 (Print)
IS  - 0273-2289 (Linking)
VI  - 112
IP  - 3
DP  - 2004 Mar
TI  - Improvement in thermal stability and substrate binding of pig kidney D-amino acid 
      oxidase by chemical modification.
PG  - 123-31
AB  - Chemical modification was evaluated to stabilize pig kidney D-amino acid oxidase 
      (pkDAAO), which is required for analytical determination of D-amino acids. 
      Optimization of modification conditions was performed to obtain high recovery 
      yield and stability, and chemical modification at 30 degrees C for 12 h with a 
      highly concentrated enzyme solution gave dextran-conjugated pkDAAO with a 70% 
      yield of activity. pkDAAO was stable at less than 55 degrees C at pH 6.0, while 
      the conjugated enzyme was stable even at 70 degrees C. In addition, the 
      conjugated enzyme showed decreased Km values for D-amino acids. Because of these 
      outstanding characteristics, this new material is expected to be available for 
      use as a liquid assay reagent.
FAU - Bakke, Mikio
AU  - Bakke M
AD  - Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, 
      Chiba 278-0037, Japan. mbakke@mail.kikkoman.co.jp
FAU - Kajiyama, Naoki
AU  - Kajiyama N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Amino Acids)
RN  - 0 (Dextrans)
RN  - 146-14-5 (Flavin-Adenine Dinucleotide)
RN  - EC 1.4.3.3 (D-Amino-Acid Oxidase)
SB  - IM
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Chromatography, Gel
MH  - D-Amino-Acid Oxidase/*chemistry/metabolism
MH  - Dextrans/*chemistry
MH  - Enzyme Stability
MH  - Flavin-Adenine Dinucleotide/metabolism
MH  - Hot Temperature
MH  - Hydrogen-Ion Concentration
MH  - Kidney/enzymology
MH  - Kinetics
MH  - Oxidation-Reduction
MH  - Protein Binding
MH  - Substrate Specificity
MH  - Swine
MH  - Temperature
EDAT- 2004/03/10 05:00
MHDA- 2004/07/14 05:00
CRDT- 2004/03/10 05:00
PHST- 2003/08/08 00:00 [received]
PHST- 2003/08/20 00:00 [revised]
PHST- 2003/09/02 00:00 [accepted]
PHST- 2004/03/10 05:00 [pubmed]
PHST- 2004/07/14 05:00 [medline]
PHST- 2004/03/10 05:00 [entrez]
AID - ABAB:112:3:123 [pii]
AID - 10.1385/abab:112:3:123 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2004 Mar;112(3):123-31. doi: 10.1385/abab:112:3:123.