PMID- 15008402
OWN - NLM
STAT- MEDLINE
DCOM- 20040805
LR  - 20181113
IS  - 0022-2844 (Print)
IS  - 0022-2844 (Linking)
VI  - 57 Suppl 1
DP  - 2003
TI  - Molecular evolution of a plastid tandem repeat locus in an orchid lineage.
PG  - S41-9
AB  - The molecular evolution of a chloroplast minisatellite locus in the Anacamptis 
      palustris (Orchidaceae) lineage and haplotype variation in two Italian A. 
      palustris populations were investigated. A phylogenetic analyses of the 
      chloroplast tRNA(LEU) intron, where the minisatellite locus is located, revealed 
      that a deletion in the ancestor of the A. palustris lineage led to the formation 
      of two noncontiguous, complementary sequence motifs. We propose a model to 
      explain the initial formation of the minisatellite repeat motif, starting with 
      the two noncontiguous, complementary sequence motifs. A survey of minisatellite 
      variation in four species of the A. palustris lineage revealed several haplotypes 
      that differed not only in repeat number, but also in repeat organization. A 
      haplotype network suggests that three different minisatellite loci evolved 
      independently at the same position in the tRNA(LEU) intron. A secondary structure 
      model revealed that the A. palustris minisatellite repeat forms a stem region of 
      the tRNA(LEU) intron, which allows its notable expansion without negatively 
      affecting splicing. Minisatellite variation was high in the two examined A. 
      palustris populations where 20 haplotypes were detected, whereas no length 
      variation was detected in a neighboring poly (A) microsatellite locus. We 
      estimated a chloroplast minisatellite mutation rate of 3.2 x 10(-3) mutations per 
      generation. Southern blot analyses did not find evidence for chloroplast 
      heteroplasmy. Based on the analysis of the largest known, extant A. palustris 
      population, a stepwise mutation model (SMM) was inferred.
FAU - Cozzolino, Salvatore
AU  - Cozzolino S
AD  - Dipartimento di Biologia Vegetale, Universita degli Studi di Napoli Federico II, 
      via Foria 223, 1-80139 Naples, Italy. cozzolin@unina.it
FAU - Cafasso, Donata
AU  - Cafasso D
FAU - Pellegrino, Giuseppe
AU  - Pellegrino G
FAU - Musacchio, Aldo
AU  - Musacchio A
FAU - Widmer, Alex
AU  - Widmer A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Mol Evol
JT  - Journal of molecular evolution
JID - 0360051
RN  - 0 (RNA, Transfer, Leu)
SB  - IM
MH  - *Evolution, Molecular
MH  - Genetic Variation
MH  - Haplotypes/genetics
MH  - Minisatellite Repeats
MH  - Models, Genetic
MH  - Mutation
MH  - Nucleic Acid Conformation
MH  - Orchidaceae/*genetics
MH  - Phylogeny
MH  - Plastids/*genetics
MH  - RNA, Transfer, Leu
MH  - *Tandem Repeat Sequences
EDAT- 2004/03/11 05:00
MHDA- 2004/08/06 05:00
CRDT- 2004/03/11 05:00
PHST- 2004/03/11 05:00 [pubmed]
PHST- 2004/08/06 05:00 [medline]
PHST- 2004/03/11 05:00 [entrez]
AID - 10.1007/s00239-003-0006-3 [doi]
PST - ppublish
SO  - J Mol Evol. 2003;57 Suppl 1:S41-9. doi: 10.1007/s00239-003-0006-3.