PMID- 15009520
OWN - NLM
STAT- MEDLINE
DCOM- 20040415
LR  - 20190906
IS  - 0022-3484 (Print)
IS  - 0022-3484 (Linking)
VI  - 39
IP  - 2
DP  - 2004 Apr
TI  - Subcutaneous and continuous administration of lipopolysaccharide increases serum 
      levels of triglyceride and monocyte chemoattractant protein-1 in rats.
PG  - 120-8
AB  - OBJECTIVES: Previous studies have suggested an association between chronic 
      infection and cardiovascular disease, and combined infection/inflammation is 
      frequently related with hypertriglyceridemia, which may be a risk factor of 
      cardiovascular disease. In fact, experimental transient or intermittent 
      administration of lipopolysaccharide (LPS) is known to cause 
      hypertriglyceridemia. We investigated the effects of subcutaneous and continuous 
      administration of LPS in rats, which was considered to mimic chronic infection 
      such as periodontal disease, on the serum levels of lipids [triglyceride (TG), 
      total cholesterol (TC) and free fatty acid (FFA)] and cytokines [tumor necrosis 
      factor-alpha (TNF-alpha) and monocyte chemoattractant protein-1 (MCP-1)], all of 
      which are thought to be etiological factors of atherosclerotic cardiovascular 
      disease. METHODS: Ten fasted Wistar rats were subcutaneously injected with LPS 
      (500 micro g/100 g of body weight) (LPS-injected rats; n = 5) or saline 
      (saline-injected rats; n = 5) as a model of transient infection. Further, 
      mini-osmotic pumps containing the same concentration of LPS (LPS-implanted rats; 
      n = 5) or saline (saline-implanted rats; n = 5) were subcutaneously implanted 
      into an additional 10 rats as a model of chronic infection. After the injection 
      or implantation, time-dependent changes in serum levels of TG, TC, FFA, 
      TNF-alpha, MCP-1 and LPS were determined. RESULTS: LPS was detected in serum 
      until 24 h in LPS-injected rats and until day 5 in LPS-implanted rats. Serum TG 
      levels significantly increased from 6 to 24 h in LPS-injected rats and from day 5 
      to 14 in LPS-implanted rats. In both LPS-injected and LPS-implanted rats, serum 
      FFA levels increased only slightly, whereas serum TC levels did not appreciably 
      change. Both types of LPS administration increased serum TNF-alpha levels 
      transiently and MCP-1 levels continuously. CONCLUSIONS: These findings suggest 
      that chronic infection such as periodontal disease induces hypertriglyceridemia 
      and increases serum MCP-1 levels in a manner that increases the risk of 
      atherosclerotic cardiovascular disease.
FAU - Uchiumi, D
AU  - Uchiumi D
AD  - Department of Periodontology, Showa University Dental School, Tokyo, Japan.
FAU - Kobayashi, M
AU  - Kobayashi M
FAU - Tachikawa, T
AU  - Tachikawa T
FAU - Hasegawa, K
AU  - Hasegawa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (Chemokine CCL2)
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Triglycerides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Body Weight
MH  - Chemokine CCL2/*blood
MH  - Cholesterol/blood
MH  - Disease Models, Animal
MH  - Fatty Acids, Nonesterified/blood
MH  - Hepatocytes/pathology
MH  - Infusion Pumps
MH  - Injections, Subcutaneous
MH  - Lipopolysaccharides/*administration & dosage/blood
MH  - Liver/pathology
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Sodium Chloride
MH  - Time Factors
MH  - Triglycerides/*blood
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 2004/03/11 05:00
MHDA- 2004/04/16 05:00
CRDT- 2004/03/11 05:00
PHST- 2004/03/11 05:00 [pubmed]
PHST- 2004/04/16 05:00 [medline]
PHST- 2004/03/11 05:00 [entrez]
AID - 716 [pii]
AID - 10.1111/j.1600-0765.2004.00716.x [doi]
PST - ppublish
SO  - J Periodontal Res. 2004 Apr;39(2):120-8. doi: 10.1111/j.1600-0765.2004.00716.x.