PMID- 15009808
OWN - NLM
STAT- MEDLINE
DCOM- 20041104
LR  - 20190827
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 63
IP  - 4
DP  - 2004 Apr
TI  - Association of polymorphic MHC microsatellites with GVHD, survival, and leukemia 
      relapse in unrelated hematopoietic stem cell transplant donor/recipient pairs 
      matched at five HLA loci.
PG  - 362-8
AB  - In order to determine whether matching/mismatching for microsatellite 
      polymorphism provides useful information on acute graft-vs-host disease (GVHD), 
      survival, and leukemia relapse in hematopoietic stem cell (HSC) transplantation, 
      we genotyped for polymorphisms at 13 microsatellite loci within the major 
      histocompatibility complex (MHC) of 100 unrelated HSC transplant donor-recipient 
      pairs who were matched at five classical human leukocyte antigen (HLA) loci. A 
      high percentage of allele matching was obtained for five microsatellite loci, 
      DQCARII (96%), MICA (93%), MIB (89%), C1-3-1 (93%), and D6S510 (97%), that are 
      localized within 100 kb of the HLA-DR, HLA-DQ, HLA-B, HLA-C, or HLA-A locus. In 
      contrast, the other eight microsatellites are located farther away from the HLA 
      classical loci and have much lower percentages of allele matching [e.g. tumor 
      necrosis factor a (TNFa) (73%), TNFd (74%), D6S273 (64%), C3-2-11 (46%), C5-3-1 
      (50%), C5-4-5 (63%), C5-2-7 (68%), and D6S265 (81%)]. Therefore, there were at 
      least eight microsatellite markers with relatively high percentages of mismatches 
      in the donor/recipient pairs with acute or chronic GVHD, poor graft survival, and 
      leukemia relapse. However, there were no statistically significant associations 
      between mismatched donor-recipient pairs at the 13 microsatellite loci and acute 
      or chronic GVHD, graft survival, and leukemia relapse. Nevertheless, allele 
      matching at the microsatellite TNFd locus near the TNFa gene was found by the 
      Fisher's exact double-sided test to be significantly associated with decreased 
      survival in the grade III/IV acute GVHD group. Overall, these results suggest 
      that the matching of microsatellite polymorphisms within the HLA region, 
      especially the ones farthest from the classical HLA loci, was not useful 
      indicator for the outcome of HSC transplantation from unrelated donors. In this 
      regard, the future determination of the genome-wide microsatellite genotypes in 
      HLA-matched donor-recipient pairs, outside the MHC, may be a better possibility 
      for identifying minor histocompatibility genes in linkage disequilibria with 
      microsatellites as potential predictive markers for the occurrence of acute GVHD 
      and survival rate in HSC transplantation.
FAU - Li, S
AU  - Li S
AD  - Department of Molecular Life Science, Division of Basic Science and Molecular 
      Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
FAU - Kawata, H
AU  - Kawata H
FAU - Katsuyama, Y
AU  - Katsuyama Y
FAU - Ota, M
AU  - Ota M
FAU - Morishima, Y
AU  - Morishima Y
FAU - Mano, S
AU  - Mano S
FAU - Kulski, J K
AU  - Kulski JK
FAU - Naruse, T
AU  - Naruse T
FAU - Inoko, H
AU  - Inoko H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Genetic Markers)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Genetic Markers
MH  - Graft vs Host Disease/*genetics/immunology/mortality
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DR Antigens/*genetics
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Histocompatibility Testing
MH  - Humans
MH  - Infant
MH  - Leukemia/genetics/immunology/pathology/*therapy
MH  - Male
MH  - Microsatellite Repeats/*genetics
MH  - Middle Aged
MH  - Prognosis
MH  - Recurrence
EDAT- 2004/03/11 05:00
MHDA- 2004/11/05 09:00
CRDT- 2004/03/11 05:00
PHST- 2004/03/11 05:00 [pubmed]
PHST- 2004/11/05 09:00 [medline]
PHST- 2004/03/11 05:00 [entrez]
AID - 200 [pii]
AID - 10.1111/j.0001-2815.2004.00200.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2004 Apr;63(4):362-8. doi: 10.1111/j.0001-2815.2004.00200.x.