PMID- 15010368
OWN - NLM
STAT- MEDLINE
DCOM- 20040827
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 104
IP  - 1
DP  - 2004 Jul 1
TI  - Reconstitution dynamics of plasmacytoid and myeloid dendritic cell precursors 
      after allogeneic myeloablative hematopoietic stem cell transplantation.
PG  - 281-9
AB  - Dendritic cells (DCs) are fundamental for immunity. We investigated 
      reconstitution of plasmacytoid DC (PDC) and myeloid DC (My-DC) precursors in the 
      first 2 months after allogeneic hematopoietic stem cell transplantation 
      (Allo-HSCT). Circulating DCs were monitored from the earliest phase of 
      hematopoietic reconstitution in 43 children given standard therapy to prevent 
      graft-versus-host disease (GVHD) and either treated or untreated with granulocyte 
      colony-stimulating factor (G-CSF) after HSCT. In patients without GVHD, both 
      My-DCs and PDCs reached consistently high absolute values during the initial 
      phase. Time of engraftment did not differ between My-DCs and PDCs, regardless of 
      administration of G-CSF. Treatment with G-CSF (1) accelerated early recovery of 
      My-DC absolute numbers; (2) was associated with lower numbers of both My-DCs and 
      PDCs in the later phase; and (3) significantly reduced the proportion of 
      interleukin-12 (IL-12)-secreting cells. In some patients who developed acute 
      GVHD, we found high numbers of circulating DC precursors during the early phase 
      of this complication. However, treatment with steroids invariably induced rapid 
      decrease of PDCs. Altogether, these data provide an evaluation of DC release 
      after Allo-HSCT, indicate that postgrafting administration of G-CSF impairs the 
      appearance of IL-12-producing DCs, and suggest that DC homeostasis may be 
      disrupted at onset of GVHD.
FAU - Fagnoni, Francesco F
AU  - Fagnoni FF
AD  - Dipartimento di Oncologia, Fondazione S. Maugeri, Istituto di Ricovero e Cura a 
      Carattere Scientifico Clinica del Lavoro e della Riabilitazione, Istituto 
      Scientifico di Pavia, Italy. ffagnoni@fsm.it
FAU - Oliviero, Barbara
AU  - Oliviero B
FAU - Giorgiani, Giovanna
AU  - Giorgiani G
FAU - De Stefano, Piero
AU  - De Stefano P
FAU - Deho, Anna
AU  - Deho A
FAU - Zibera, Carlo
AU  - Zibera C
FAU - Gibelli, Nadia
AU  - Gibelli N
FAU - Maccario, Rita
AU  - Maccario R
FAU - Da Prada, GianAntonio
AU  - Da Prada G
FAU - Zecca, Marco
AU  - Zecca M
FAU - Locatelli, Franco
AU  - Locatelli F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040309
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Steroids)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Adolescent
MH  - Antigens, CD/immunology
MH  - Case-Control Studies
MH  - Cell Differentiation
MH  - Child
MH  - Child, Preschool
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*cytology/immunology/ultrastructure
MH  - Female
MH  - Graft vs Host Disease/blood/drug therapy/immunology
MH  - Granulocyte Colony-Stimulating Factor/therapeutic use
MH  - Hematopoietic Stem Cell Transplantation/*adverse effects/methods
MH  - Humans
MH  - Leukocyte Count
MH  - Lipopolysaccharides/immunology/pharmacology
MH  - Male
MH  - Myeloid Cells/*cytology/immunology/ultrastructure
MH  - Plasma Cells/*cytology/immunology/ultrastructure
MH  - Postoperative Complications/blood/drug therapy/immunology
MH  - Prospective Studies
MH  - Steroids/therapeutic use
MH  - Time Factors
MH  - Transplantation Conditioning/*adverse effects/methods
MH  - Transplantation, Homologous
MH  - Treatment Outcome
EDAT- 2004/03/11 05:00
MHDA- 2004/08/28 05:00
CRDT- 2004/03/11 05:00
PHST- 2004/03/11 05:00 [pubmed]
PHST- 2004/08/28 05:00 [medline]
PHST- 2004/03/11 05:00 [entrez]
AID - S0006-4971(20)54537-0 [pii]
AID - 10.1182/blood-2003-07-2443 [doi]
PST - ppublish
SO  - Blood. 2004 Jul 1;104(1):281-9. doi: 10.1182/blood-2003-07-2443. Epub 2004 Mar 9.